Hyaluronan/colistin polyelectrolyte complexes: Promising antiinfective drug delivery systems

被引:24
作者
Dubashynskaya, Natallia, V [1 ]
Raik, Sergei, V [1 ]
Dubrovskii, Yaroslav A. [2 ,3 ,4 ]
Shcherbakova, Elena S. [5 ]
Demyanova, Elena, V [5 ]
Shasherina, Anna Y. [2 ]
Anufrikov, Yuri A. [2 ]
Poshina, Daria N. [1 ]
Dobrodumov, Anatoliy, V [1 ]
Skorik, Yury A. [1 ]
机构
[1] Russian Acad Sci, Inst Macromol Cpds, Bolshoi VO 31, St Petersburg 199004, Russia
[2] St Petersburg State Univ, Inst Chem, Univ Skii 26, St Petersburg 198504, Russia
[3] Almazov Natl Med Res Ctr, Akkuratova 2, St Petersburg 197341, Russia
[4] St Petersburg State Chem Pharmaceut Univ, Prof Popova 14, St Petersburg 197376, Russia
[5] State Res Inst Highly Pure Biopreparat, Pudozhsakya 7, St Petersburg 197110, Russia
基金
俄罗斯科学基金会;
关键词
Colistin; Hyaluronic acid; Drug delivery system; HYALURONIC-ACID; COLISTIN; NANOPARTICLES; INFECTIONS; PEPTIDES; FUNCTIONALIZATION; NANOCARRIERS; ANTIBIOTICS; POLYMYXINS; RESISTANCE;
D O I
10.1016/j.ijbiomac.2021.07.114
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nanotechnology-based modification of known antimicrobial agents is a rational and straightforward way to improve their safety and effectiveness. The aim of this study was to develop colistin (CT)-loaded polymeric carriers based on hyaluronic acid (HA) for potential application as antimicrobial agents against multi-resistant gram-negative microorganisms (including ESKAPE pathogens). CT-containing particles were obtained via a polyelectrolyte interaction between protonated CT amino groups and HA carboxyl groups (the CT-HA complex formation constant [log(CT-)(HA)] was about 5.0). The resulting polyelectrolyte complexes had a size of 210-250 nm and a negative charge (zeta-potential -19 mV), with encapsulation and loading efficiencies of 100% and 20%, respectively. The developed CT delivery systems were characterized by modified release (45% and 85% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro tests showed that the encapsulation of CT in polymer particles did not reduce its pharmacological activity; the minimum inhibitory concentrations of both encapsulated CT and pure CT were 1 mu g/mL (against Pseudomonas aeruginosa).
引用
收藏
页码:157 / 165
页数:9
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