The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates

被引:54
作者
Edwards, Marcus J. [1 ,2 ]
Hall, Andrea [1 ,2 ]
Shi, Liang [3 ]
Fredrickson, James K. [3 ]
Zachara, John M. [3 ]
Butt, Julea N. [1 ,2 ]
Richardson, David J. [1 ,2 ]
Clarke, Thomas A. [1 ,2 ]
机构
[1] Univ E Anglia, Sch Biol Sci, Ctr Mol & Struct Biochem, Norwich NR4 7TJ, Norfolk, England
[2] Univ E Anglia, Sch Chem, Norwich NR4 7TJ, Norfolk, England
[3] Pacific NW Natl Lab, Richland, WA 99352 USA
基金
英国生物技术与生命科学研究理事会;
关键词
C-TYPE CYTOCHROME; ELECTRON-TRANSFER; SHEWANELLA; OMCA; MTRC; REDUCTION; SURFACE; FE(III);
D O I
10.1016/j.str.2012.04.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Members of the genus Shewanella translocate deca-or undeca-heme cytochromes to the external cell surface thus enabling respiration using extracellular minerals and polynuclear Fe(III) chelates. The high resolution structure of the first undeca-heme outer membrane cytochrome, UndA, reveals a crossed heme chain with four potential electron ingress/egress sites arranged within four domains. Sequence and structural alignment of UndA and the deca-heme MtrF reveals the extra heme of UndA is inserted between MtrF hemes 6 and 7. The remaining UndA hemes can be superposed over the heme chain of the decaheme MtrF, suggesting that a ten heme core is conserved between outer membrane cytochromes. The UndA structure has also been crystal-lographically resolved in complex with substrates, an Fe(III)-nitrilotriacetate dimer or an Fe(III)-citrate trimer. The structural resolution of these UndA-Fe(III)-chelate complexes provides a rationale for previous kinetic measurements on UndA and other outer membrane cytochromes.
引用
收藏
页码:1275 / 1284
页数:10
相关论文
共 31 条
[11]   Characterization of an electron conduit between bacteria and the extracellular environment [J].
Hartshorne, Robert S. ;
Reardon, Catherine L. ;
Ross, Daniel ;
Nuester, Jochen ;
Clarke, Thomas A. ;
Gates, Andrew J. ;
Mills, Paul C. ;
Fredrickson, Jim K. ;
Zachara, John M. ;
Shi, Liang ;
Beliaev, Alex S. ;
Marshall, Matthew J. ;
Tien, Ming ;
Brantley, Susan ;
Butt, Julea N. ;
Richardson, David J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (52) :22169-22174
[12]   CRYSTAL AND MOLECULAR-STRUCTURE OF A NEW MU-OXO-BRIDGED IRON(III) DIMER FORMED WITH THE NITRILOTRIACETATE LIGAND [J].
HEATH, SL ;
POWELL, AK ;
UTTING, HL ;
HELLIWELL, M .
JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS, 1992, (02) :305-307
[13]   Characterization of the Decaheme c-Type Cytochrome OmcA in Solution and on Hematite Surfaces by Small Angle X-Ray Scattering and Neutron Reflectometry [J].
Johs, A. ;
Shi, L. ;
Droubay, T. ;
Ankner, J. F. ;
Liang, L. .
BIOPHYSICAL JOURNAL, 2010, 98 (12) :3035-3043
[14]   Integration of macromolecular diffraction data [J].
Leslie, AGW .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1999, 55 :1696-1702
[15]   Specific bonds between an iron oxide surface and outer membrane cytochromes MtrC and OmcA from Shewanella oneidensis MR-1 [J].
Lower, Brian H. ;
Shi, Liang ;
Yongsunthon, Ruchirej ;
Droubay, Timothy C. ;
McCready, David E. ;
Lower, Steven K. .
JOURNAL OF BACTERIOLOGY, 2007, 189 (13) :4944-4952
[16]   Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1 [J].
Lower, Brian H. ;
Yongsunthon, Ruchirej ;
Shi, Liang ;
Wildling, Linda ;
Gruber, Hermann J. ;
Wigginton, Nicholas S. ;
Reardon, Catherine L. ;
Pinchuk, Grigoriy E. ;
Droubay, Timothy C. ;
Boily, Jean-Francois ;
Lower, Steven K. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (09) :2931-2935
[17]   Phaser crystallographic software [J].
McCoy, Airlie J. ;
Grosse-Kunstleve, Ralf W. ;
Adams, Paul D. ;
Winn, Martyn D. ;
Storoni, Laurent C. ;
Read, Randy J. .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2007, 40 :658-674
[18]   Oxygen-dependent autoaggregation in Shewanella oneidensis MR-1 [J].
McLean, J. S. ;
Pinchuk, G. E. ;
Geydebrekht, O. V. ;
Bilskis, C. L. ;
Zakrajsek, B. A. ;
Hill, E. A. ;
Saffarini, D. A. ;
Romine, M. F. ;
Gorby, Y. A. ;
Fredrickson, J. K. ;
Beliaev, A. S. .
ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (07) :1861-1876
[19]   Breaking good resolutions with ARP/wARP [J].
Morris, RJ ;
Zwart, PH ;
Cohen, S ;
Fernandez, FJ ;
Kakaris, M ;
Kirillova, O ;
Vonrhein, C ;
Perrakis, A ;
Lamzin, VS .
JOURNAL OF SYNCHROTRON RADIATION, 2004, 11 :56-59
[20]   REFMAC5 for the refinement of macromolecular crystal structures [J].
Murshudov, Garib N. ;
Skubak, Pavol ;
Lebedev, Andrey A. ;
Pannu, Navraj S. ;
Steiner, Roberto A. ;
Nicholls, Robert A. ;
Winn, Martyn D. ;
Long, Fei ;
Vagin, Alexei A. .
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2011, 67 :355-367