DEVELOPMENT OF EST-SSR MARKERS FOR ELAEOCARPUS PHOTINIIFOLIA (ELAEOCARPACEAE), AN ENDEMIC TAXON OF THE BONIN ISLANDS

被引：4

作者：

Sugai, Kyoko ^{[1
]}

Setsuko, Suzuki ^{[2
]}

Uchiyama, Kentaro ^{[2
]}

Murakami, Noriaki ^{[1
]}

Kato, Hidetoshi ^{[1
]}

Yoshimaru, Hiroshi ^{[2
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Sci & Engn, Makino Herbarium, Hachioji, Tokyo 1920397, Japan

[2] Forestry & Prod Res Inst, Dept Forest Genet, Tsukuba, Ibaraki 3058786, Japan

来源：

AMERICAN JOURNAL OF BOTANY | 2012年 / 99卷 / 02期

基金：

日本学术振兴会;

关键词：

Bonin (Ogasawara) Islands; Elaeocarpaceae; Elaeocarpus photiniifolia; expressed sequence tags; microsatellite; next-generation sequencing; simple sequence repeat markers;

D O I：

10.3732/ajb.1100391

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Expressed sequence tag (EST) - derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands. Methods and Results: Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A total of 267 primer pairs were designed from the library. Of the 48 tested loci, 25 loci were polymorphic among 41 individuals representing the entire geographical range of the species, with the number of alleles per locus and expected heterozygosity ranging from two to 14 and 0.09 to 0.86, respectively. Most loci were transferable to a related species, E. sylvestris. Conclusions: The developed markers will be useful for evaluating the genetic structure of E. photiniifolia.

引用

页码：E84 / E87

页数：4

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