Planar Airy beam light-sheet for two-photon microscopy

被引：34

作者：

Hosny, Neveen A. ^{[1
]}

Seyforth, James A. ^{[1
]}

Spickermann, Gunnar ^{[1
]}

Mitchell, Thomas J. ^{[1
]}

Almada, Pedro ^{[1
]}

Chesters, Robert ^{[2
]}

Mitchell, Scott J. ^{[2
,3
]}

Chennell, George ^{[2
]}

Vernon, Anthony C. ^{[2
,4
]}

Cho, Kwangwook ^{[2
,3
]}

Srivastava, Deepak P. ^{[2
,4
]}

Forster, Robert ^{[1
]}

Vettenburg, Tom ^{[5
,6
]}

机构：

[1] Surrey Technol Ctr, M Squared Life, Guildford GU2 7YG, Surrey, England

[2] Kings Coll London, Basic & Clin Neurosci Dept, London SE5 9NU, England

[3] Kings Coll London, UK Dementia Res Inst, London, England

[4] Kings Coll London, MRC Ctr Neurodev Disorders, London, England

[5] Univ Exeter, Sch Phys & Astron, Exeter EX4 4QL, Devon, England

[6] Univ Dundee, Sch Sci & Engn, Dundee DD1 4HN, Scotland

来源：

BIOMEDICAL OPTICS EXPRESS | 2020年 / 11卷 / 07期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国科研创新办公室;

关键词：

DEEP;

D O I：

10.1364/BOE.395547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.

引用

页码：3927 / 3935

页数：9

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