Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urine

被引：40

作者：

He, Qiyi ^{[1
]}

Chen, Yingshan ^{[1
]}

Shen, Ding ^{[1
]}

Cui, Xiping ^{[1
]}

Zhang, Chunguo ^{[1
]}

Yang, Huiyi ^{[1
]}

Zhong, Wenying ^{[1
]}

Eremin, Sergei A. ^{[2
,3
]}

Fang, Yanxiong ^{[1
]}

Zhao, Suqing ^{[1
]}

机构：

[1] Guangdong Univ Technol, Fac Chem Engn & Light Ind, Dept Pharmaceut Engn, Guangzhou 510006, Guangdong, Peoples R China

[2] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia

[3] Natl Res Tech Univ MISiS, Moscow 119049, Russia

来源：

TALANTA | 2019年 / 195卷

关键词：

3-nitrotyrosine; Surface plasmon resonance (SPR); ELISA; Human urine; SENSITIVE DETERMINATION; NITROTYROSINE; BIOSENSOR; PROTEIN; IMMUNOASSAY; AUTOMATION; EXTRACTION; NITRATION; ANTIBODY; ASSAY;

D O I：

10.1016/j.talanta.2018.11.110

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this paper, on the basis of anti-3-NT specific antibody, we developed a label-free indirect competitive surface plasmon resonance (SPR) immunosensor and ELISA for the detection of 3-NT. Under the optimized conditions, the SPR immunosensor can obtain a linear range of 0.17-6.07 mu g/mL and a limit of detection (LOD) of 0.12 mu g/mL while the ELISA can reach 0.33-9.94 mu g/mL and a LOD of 0.24 mu g/mL.The selectivity of 3-NT was also testified by six kinds of amino acid analogues. Besides, the developed SPR immunosensor was compared thoroughly with a conventional ELISA in spiked analysis of urine samples. Good recoveries and correlation between these two methods were observed (R-2 = 0.964). Therefore, it is concluded that the automated SPR platform can be applied to quantify 3-NT in biological samples with its sensitivity, accuracy, and real timing.

引用

页码：655 / 661

页数：7

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