High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry

被引：96

作者：

Chen, Rui ^{[1
]}

Xu, Wenjun ^{[1
]}

Xiong, Caiqiao ^{[1
]}

Zhou, Xiaoyu ^{[1
]}

Xiong, Shaoxiang ^{[1
]}

Nie, Zongxiu ^{[1
]}

Mao, Lanqun ^{[1
]}

Chen, Yi ^{[1
]}

Chang, Huan-Cheng ^{[2
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China

[2] Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 01期

关键词：

CAPILLARY-ELECTROPHORESIS; ELECTROCHEMICAL METHOD; CEREBROSPINAL-FLUID; GOLD NANOPARTICLES; ONLINE; LACTATE; IONIZATION; MS;

D O I：

10.1021/ac202438a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Due to its strong ultraviolet absorption, high salt tolerance, and little interference in the low molecular weight region, N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) has been applied as a matrix to measure the level of glucose in rat brain microdialysates by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in combination with in vivo microdialysis. By monitoring the ion signals of (glucose + C1)(-) in the mass spectra, we achieved a low detection limit of similar to 10 mu M for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification. It is concluded that NEDC-assisted laser desorption/ionization (LDI) MS is a fast and general method for sensitive detection of small molecules (such as glucose and amino adds) in high ionic strength solutions.

引用

页码：465 / 469

页数：5

共 30 条

[1]

[Anonymous], 1988, Rapid Commun. Mass Spectrom, DOI [DOI 10.1002/RCM.1290020802, 10.1002/rcm.1290020802]

[2] Screening of microdialysates taken before and after induced liver damage;: on-line solid phase extraction-electrospray ionization-mass spectrometry [J].

Bergström, SK ;

Goiny, M ;

Danielsson, R ;

Ungerstedt, U ;

Andersson, M ;

Markides, KE .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1120 (1-2) :21-26

[3]

Börnsen KO, 2000, METH MOL B, V146, P387, DOI 10.1385/1-59259-045-4:387

[4] Stabilization of anionic adducts in negative ion electrospray mass spectrometry [J].

Cai, Y ;

Cole, RB .

ANALYTICAL CHEMISTRY, 2002, 74 (05) :985-991

[5]

Chen H., 2007, Angew. Chem, V119, P7735, DOI DOI 10.1002/ANGE.200702200

[6] Small molecule analysis by MALDI mass spectrometry [J].

Cohen, LH ;

Gusev, AI .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 373 (07) :571-586

[7] Perspectives in imaging using mass spectrometry [J].

Dill, Allison L. ;

Eberlin, Livia S. ;

Ifa, Demian R. ;

Cooks, R. Graham .

CHEMICAL COMMUNICATIONS, 2011, 47 (10) :2741-2746

[8] Metabolite identification in rat brain microdialysates by direct infusion nanoelectrospray ionization after desalting on a ZipTip and LTQ/Orbitrap mass spectrometry [J].

Erve, John C. L. ;

Beyer, Chad E. ;

Manzino, Lawrence ;

Talaat, Rasmy E. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (24) :4003-4012

[9] Determination of baicalin in rat cerebrospinal fluid and blood using microdialysis coupled with ultra-performance liquid chromatography-tandem mass spectrometry [J].

Huang, Huifeng ;

Zhang, Yu ;

Yang, Rui ;

Tang, Xing .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2008, 874 (1-2) :77-83

[10] Colorimetric Detection of Glucose in Rat Brain Using Gold Nanoparticles [J].

Jiang, Ying ;

Zhao, Hong ;

Lin, Yuqing ;

Zhu, Ningning ;

Ma, Yurong ;

Mao, Lanqun .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (28) :4800-4804

← 1 2 3 →