Activation of Toll-like receptor 2 induces B1 and B2 kinin receptors in human gingival fibroblasts and in mouse gingiva

被引：10

作者：

Souza, Pedro P. C. ^{[1
,5
]}

Lundberg, Pernilla ^{[2
]}

Lundgren, Inger ^{[2
]}

Magalhaes, Fernando A. C. ^{[1
]}

Costa-Neto, Claudio M. ^{[3
]}

Lerner, Ulf H. ^{[2
,4
]}

机构：

[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazil

[2] Umea Univ, Dept Mol Periodontol, Umea, Sweden

[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, SP, Brazil

[4] Univ Gothenburg, Sahlgrenska Acad, Inst Med, Dept Internal Med & Clin Nutr,Ctr Bone & Arthrit, Gothenburg, Sweden

[5] Fed Univ Goias UFG, Sch Dent, Goiania, Go, Brazil

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

NF-KAPPA-B; ACTIVE RHEUMATOID-ARTHRITIS; NECROSIS-FACTOR-ALPHA; PORPHYROMONAS-GINGIVALIS; BONE-RESORPTION; UP-REGULATION; PROSTAGLANDIN BIOSYNTHESIS; CUTTING EDGE; LIPOPOLYSACCHARIDE; BRADYKININ;

D O I：

10.1038/s41598-018-37777-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The regulation of the kallikrein-kinin system is an important mechanism controlling vasodilation and promoting inflammation. We aimed to investigate the role of Toll-like receptor 2 (TLR2) in regulating kinin B-1 and B-2 receptor expression in human gingival fibroblasts and in mouse gingiva. Both P. gingivalis LPS and the synthetic TLR2 agonist Pam(2)CSK(4) increased kinin receptor transcripts. Silencing of TLR2, but not of TLR4, inhibited the induction of kinin receptor transcripts by both P. gingivalis LPS and Pam(2)CSK(4). Human gingival fibroblasts (HGF) exposed to Pam(2)CSK(4) increased binding sites for bradykinin (BK, B-2 receptor agonist) and des-Arg(10)-Lys-bradykinin (DALBK, B-1 receptor agonist). Pre-treatment of HGF for 24 h with Pam(2)CSK(4) resulted in increased PGE(2) release in response to BK and DALBK. The increase of B-1 and B-2 receptor transcripts by P. gingivalis LPS was not blocked by IL-1 beta neutralizing antibody; TNF-alpha blocking antibody did not affect B-1 receptor up-regulation, but partially blocked increase of B-2 receptor mRNA. Injection of P. gingivalis LPS in mouse gingiva induced an increase of B-1 and B-2 receptor mRNA. These data show that activation of TLR2 in human gingival fibroblasts as well as in mouse gingival tissue leads to increase of B-1 and B-2 receptor mRNA and protein.

引用

页数：11

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