Microbiological yield from induced sputum compared to oropharyngeal swab in young children with cystic fibrosis

被引：30
作者：
Zampoli, Marco [1 ]
Pillay, Komala [2 ]
Carrara, Henri [3 ]
Zar, Heather J. [4 ,5 ]
Morrow, Brenda [1 ]
机构：
[1] Univ Cape Town, Red Cross War Mem Childrens Hosp, Div Pediat Pulmonol, Dept Paediat & Child Hlth, 5th Floor ICH Bldg,Klipfontein Rd, ZA-7700 Cape Town, South Africa
[2] Univ Cape Town, Red Cross War Mem Childrens Hosp, Dept Anat Pathol, Cape Town, South Africa
[3] Univ Cape Town, Fac Hlth Sci, Sch Publ Hlth & Family Med, Cape Town, South Africa
[4] Univ Cape Town, Red Cross War Mem Childrens Hosp, Dept Paediat & Child Hlth, Cape Town, South Africa
[5] Univ Cape Town, MRC Unit Child & Adolescent Hlth, Cape Town, South Africa
来源：
JOURNAL OF CYSTIC FIBROSIS | 2016年 / 15卷 / 05期
关键词：
Induced sputum; Oropharyngeal swab; Cystic fibrosis; Young children; BRONCHOALVEOLAR LAVAGE; PULMONARY TUBERCULOSIS; LUNG-FUNCTION; INDUCTION; INFECTION; INFANTS; PATHOGENS; CULTURES; CARE; INFLAMMATION;
D O I：
10.1016/j.jcf.2016.01.001
中图分类号：
R56 [呼吸系及胸部疾病];
学科分类号：
摘要：
Background: Standard respiratory sampling in young children with cystic fibrosis (CF) is by oropharyngeal swab (OPS) as they cannot spontaneously expectorate. Sputum induction (IS) has been poorly investigated in this population. We aimed to compare the bacteriological yield of OPS vs. IS in young children with CF. Methods: Sequentially paired OPS followed by IS samples was collected in children <5 years of age attending a CF clinic in Cape Town, South Africa. Results: IS was successfully paired with OPS in 98/113 (85%) attempts in 32 children (mean +/- SD 19 +/- 16 months), with no serious adverse events. IS culture yield for any CF-associated bacteria from IS was 46% vs. 28% from OPS (p = 0.01). The sensitivity, specificity, PPV and NPV of OPS compared to IS in isolating CF-associated bacteria were 56%, 96%, 93%, and 72% respectively. Conclusion: Sputum induction is feasible, safe and superior to OPS for detecting CF-associated bacteria in young children with CF. (C) 2016 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
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收藏
页码：605 / 610
页数：6
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