Rapid determination of cinnamic acid and harpagoside in a traditional Chinese medicine of Scrophularia ningpoensis by microwave-assisted extraction followed by high performance liquid chromatography (HPLC)

被引：0
作者：
Huang, Taomin [1 ]
Chen, Nianzu [1 ]
Lai, Yonghua [1 ]
Wang, Donglei [1 ]
Yan, Jinchao [1 ]
Gu, Jifeng [1 ]
机构：
[1] Fudan Univ, Dept Pharm, Eye & ENT Hosp, Shanghai 200031, Peoples R China
来源：
JOURNAL OF MEDICINAL PLANTS RESEARCH | 2011年 / 5卷 / 08期
关键词：
Scrophularia ningpoensis; Cinnamic acid; harpagoside; microwave-assisted extraction; liquid chromatography; traditional Chinese medicine; SINGLE-DROP MICROEXTRACTION; ESSENTIAL OIL; HARPAGOPHYTUM-PROCUMBENS; MASS SPECTROMETRY; CINNAMALDEHYDE; GLYCYRRHIZIN; PAEONIFLORIN; DISTILLATION; HARPAGIDE; PUERARIN;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Cinnamic acid and harpagoside are important active components present in Scrophularia ningpoensis, which has been used in the treatment of several diseases such as pharyngalgia. In the present study, a novel method based on high performance liquid chromatography (HPLC) following microwave-assisted extraction (MAE) was developed for the determination of cinnamic acid and harpagoside in a traditional chinese medicine (TCM) of S. ningpoensis. Effective chromatographic separation was achieved on a C18 column at the detection wavelength of 278 nm. Microwave power of 400 W and irradiation time of 4 min were found to be the optimum conditions for the MAE process. In addition, to demonstrate the feasibility of the MAE-HPLC method, the conventional ultrasound extraction (USE) method was used for the analysis of cinnamic acid and harpagoside in the TCM. The results indicated that MAE-HPLC is a simple, rapid, efficient, and low cost method for the determination of cinnamic acid and harpagoside in TCM as well as a potential tool for the assessment of TCM quality.
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收藏
页码：1313 / 1320
页数：8
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