Halo reduction technique in phase contrast microscopy

被引：8

作者：

Otaki, T ^{[1
]}

机构：

[1] Nikon Inc, Instruments Co, Opt Design Dept, Shinagawa Ku, Tokyo 1408601, Japan

来源：

OPTICAL REVIEW | 2001年 / 8卷 / 04期

关键词：

phase contrast microscopy; apodization; halo; phase difference; bluish hue; apodized phase contrast;

D O I：

10.1007/s10043-001-0284-1

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A new phase contrast microscopy technique has been developed which achieves halo reduction by applying an apodization to the Zernike phase contrast method. In this technique, a new apodized phase plate is placed at the back focal plane of the objective. This plate consists of a quarter wave phase shift ring conjugated of the illuminating aperture, which has a 25% transmittance, and a pair of adjacent rings having 50% transmittance. The developed technique yields the highest resolution images without a bluish hue effect ever achieved in phase contrast microscopy.

引用

页码：284 / 286

页数：3

共 11 条

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Otaki, T
[J]. OPTICAL REVIEW, 2000, 7 (02) : 119 - 122

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