Characterization of an extracellular serine protease gene from the nematophagous fungus Lecanicillium psalliotae

被引：30

作者：

Yang, JK

Huang, XW

Tian, BY

Sun, H

Duan, JX

Wu, WP

Zhang, KQ ^{[1
]}

机构：

[1] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China

[2] Novozymes China, Ctr Res & Dev, Beijing 100085, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 17期

关键词：

gene cloning; Lecanicillium psalliotae; serine protease; similarity comparison;

D O I：

10.1007/s10529-005-0482-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene encoding a cuticle-degrading serine protease was cloned from three isolates of Lecanicillium psalliotae (syn. Verticillium psalliotae) by 3' and 5' RACE (rapid amplification of cDNA ends) method. The gene encodes for 382 amino acids and the protein shares conserved motifs with subtilisin N and peptidase S8. Comparison of translated cDNA sequences of three isolates revealed one amino acid polymorphism at position 230. The deduced protease sequence shared high degree of similarities to other cuticle-degrading proteases from other nematophagous fungi.

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页码：1329 / 1334

页数：6

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