Immunoglobulin light chain isotypes in the teleost Trematomus bernacchii

被引：22

作者：

Coscia, Maria Rosaria ^{[1
]}

Giacomelli, Stefano ^{[1
]}

De Santi, Concetta ^{[1
]}

Varriale, Sonia ^{[1
]}

Oreste, Umberto ^{[1
]}

机构：

[1] CNR, Inst Prot Biochem, I-80131 Naples, Italy

来源：

MOLECULAR IMMUNOLOGY | 2008年 / 45卷 / 11期

关键词：

immunoglobulin light chain isotypes; teleosts; Antarctic teleosts; immunoglobulin light chain structure; immunoglobulin light chain evolution; Trematomus bernacchii; real-time PCR; molecular models;

D O I：

10.1016/j.molimm.2008.03.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three immunoglobulin light chain (IgL) isotypes TrbeL1, TrbeL2, and TrbeL3 were identified in the Antarctic teleost Trematomus bernacchii by immunoscreening a cDNA expression library, and using RT-PCR, and 5' RACE. One of them was distinguished in two subisotypes TrbeL1A and TrbeL1B. Real-time PCR experiments showed that the different isotypes were expressed in similar ratios in the various tissues analyzed. Interestingly, the expression level of TrbeL1A isotype was very high in all tissues. Molecular models of the CH1-CL domain pairings were built and minimized for the different isotypes. Several differences were identified in the superimposable structures mainly in the loops. In addition, the isotype-specific residues determined a different distribution of the charges on the external CL domain surface. Phylogenetic trees of 43 isotype representative sequences of CL domain from teleost species, built by different methods, indicated that all teleost light chain isotypes are distributed into three groups. Furthermore, the split of the group IgL1 into two subgroups, one of them carrying a micro-satellite DNA insertion, may have occurred in the Acanthopterygean ancestor. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：3096 / 3106

页数：11

共 49 条

[1] ProtTest: selection of best-fit models of protein evolution [J].