Polymorphism Analysis of the Horse Dopamine Receptor D4 Gene (DRD4) Sequence

被引：0
|
作者：
Fan, Caiyun [1 ]
Cheng, Jianbo [2 ]
Li, Jinlian [3 ]
Wang, Qinghua [1 ]
Manglai, Dugarjaviin [4 ]
机构：
[1] Henan Inst Sci & Technol, Coll Anim Sci, Xinxiang 453003, Peoples R China
[2] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China
[3] Taishan Univ, Dept Biol Sci & Technol, Tai An 271021, Shandong, Peoples R China
[4] Inner Mongolia Agr Univ, Coll Anim Sci & Technol, Hohhot 010018, Peoples R China
来源：
JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2011年 / 10卷 / 14期
基金：
中国国家自然科学基金;
关键词：
Horses; DRD4; gene; polymorphism; PCR-RFLP; silver staining; local bread; China; AFFECTIVE-DISORDERS; ALLELE FREQUENCY; NOVELTY SEEKING; EXON-III; ASSOCIATION; SUSCEPTIBILITY; REGION; BREEDS;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In this study, partial sequence of horse DRD4 gene was cloned including intron 1, partial exon 1 and exon 2. And restriction endonuclease Stu I was used to analyze the polymorphism of the DRD4 gene sequences of 270 horses from six types including importing breed, cultivating breed and local breed. The products of endonuclease cutting were detected by 8% non-denatured polyacrylamide gel electrophoresis and showing in silver staining protocol. The result indicated restriction endonuclease Stu I showed polymorphism. Six kinds of genotypes were found in six populations which were controlled by three alleles. The results of Chi-square (chi(2)) test showed that genotypes of horse DRD4 gene in TB, SH, XN did not fit with Hardy-Weinberg equilibrium (p<0.05) but in WS, BH and WZ fit with Hardy-Weinberg equilibrium (p>0.05).
引用
收藏
页码：1855 / 1858
页数：4
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