Antibody response in goats experimentally infected with Toxoplasma gondii

被引：22
|
作者：
Vitor, RWA
Ferreira, AM
Fux, B
机构：
[1] UFMG, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil
[2] UFMG, Bolsista CNPq, Belo Horizonte, MG, Brazil
来源：
VETERINARY PARASITOLOGY | 1999年 / 81卷 / 03期
关键词：
goats; Toxoplasma gondii; ELISA; indirect hemagglutination; Western blotting;
D O I：
10.1016/S0304-4017(98)00251-9
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Serum samples of five goats inoculated with Toxoplasma gondii were analyzed using the enzyme linked immunosorbent assay (ELISA), indirect hemagglutination (IHA) and western blotting (WB). Antibodies detected by ELISA peaked between 19 and 62 days after inoculation and persisted throughout the experiment with no association to parasitaemia. Using WB, the main antigens detected had molecular weights of approximately 68, 62, 50, 48, 42, 34, 28, 26, 22 and 19 kDa. Antibody titers of between 1 : 256 and 1 : 32000 were observed using IHA, with a significant drop in activity after treatment with 2-mercapto-ethanol between days 12 and 48. This coincided with the parasitaemic period that occurs between 5 and 64 days after inoculation. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：259 / 263
页数：5
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