Antibody response in goats experimentally infected with Toxoplasma gondii

被引：22
|
作者：
Vitor, RWA
Ferreira, AM
Fux, B
机构：
[1] UFMG, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG, Brazil
[2] UFMG, Bolsista CNPq, Belo Horizonte, MG, Brazil
来源：
VETERINARY PARASITOLOGY | 1999年 / 81卷 / 03期
关键词：
goats; Toxoplasma gondii; ELISA; indirect hemagglutination; Western blotting;
D O I：
10.1016/S0304-4017(98)00251-9
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Serum samples of five goats inoculated with Toxoplasma gondii were analyzed using the enzyme linked immunosorbent assay (ELISA), indirect hemagglutination (IHA) and western blotting (WB). Antibodies detected by ELISA peaked between 19 and 62 days after inoculation and persisted throughout the experiment with no association to parasitaemia. Using WB, the main antigens detected had molecular weights of approximately 68, 62, 50, 48, 42, 34, 28, 26, 22 and 19 kDa. Antibody titers of between 1 : 256 and 1 : 32000 were observed using IHA, with a significant drop in activity after treatment with 2-mercapto-ethanol between days 12 and 48. This coincided with the parasitaemic period that occurs between 5 and 64 days after inoculation. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：259 / 263
页数：5
相关论文
共 50 条
  • [11] DEMONSTRATION BY MICROSCOPY OF PARASITEMIA IN ANIMALS EXPERIMENTALLY INFECTED WITH TOXOPLASMA GONDII
    ITO, S
    TSUNODA, K
    SUZUKI, K
    TSUTSUMI, Y
    NATIONAL INSTITUTE OF ANIMAL HEALTH QUARTERLY, 1966, 6 (01) : 8 - +
  • [12] Pathological and immunohistochemical studies in rabbits experimentally infected with Toxoplasma gondii
    Haziroglu, R
    Altintas, K
    Atasever, A
    Gülbahar, MY
    Kul, O
    Tunca, R
    TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2003, 27 (02): : 285 - 293
  • [13] Skeletal muscle pathology in mice experimentally infected with Toxoplasma gondii
    Tonino, P
    Finol, HJ
    Marquez, A
    JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY, 1996, 28 (04) : 521 - 526
  • [14] Toxoplasma gondii: Reproductive parameters in experimentally infected male rats
    Terpsidis, Konstantinos I.
    Papazahariadou, Margarita G.
    Taitzoglou, Ioannis A.
    Papaioannou, Nikolaos G.
    Georgiadis, Marios P.
    Theodoridis, Ioannis Th.
    EXPERIMENTAL PARASITOLOGY, 2009, 121 (03) : 238 - 241
  • [15] STUDIES ON PERITONEAL EXUDATE OF ANIMALS EXPERIMENTALLY INFECTED WITH TOXOPLASMA GONDII
    SAMIR, MA
    ACTA MICROBIOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE, 1969, 16 (01): : 39 - &
  • [16] Anatomical distribution of Toxoplasma gondii in naturally and experimentally infected lambs
    Thomas, Myriam
    Aubert, Dominique
    Escotte-Binet, Sandie
    Durand, Benoit
    Robert, Celine
    Geers, Regine
    Alliot, Annie
    Belbis, Guillaume
    Villena, Isabelle
    Blaga, Radu
    PARASITE, 2022, 29
  • [17] TOXOPLASMA-GONDII CYSTS IN PLACENTAS OF EXPERIMENTALLY INFECTED SHEEP
    DUBEY, JP
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1987, 48 (03) : 352 - 353
  • [18] Detection of Toxoplasma gondii in the milk of experimentally infected lactating cats
    Powell, CC
    Brewer, M
    Lappin, MR
    VETERINARY PARASITOLOGY, 2001, 102 (1-2) : 29 - 33
  • [19] Immunodetection of Toxoplasma gondii in placenta tissue of naturally infected goats
    Mesquita, E. P.
    Oliveira, J. M. B.
    Silva, G. M.
    Torres, S. M.
    Oliveira, A. A. F.
    Silva Junior, V. A.
    Mota, R. A.
    Amorim, M. J. A. A. L.
    ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 2019, 71 (01) : 86 - 92
  • [20] TOXOPLASMA-GONDII HEMAGGLUTINATING ANTIBODY TITERS IN INDONESIAN GOATS
    CROSS, JH
    VANPEENEN, PFD
    HSU, NHM
    KOESHARJONO, C
    SIMANJUNTAK, GM
    AMDANI, SK
    TROPICAL AND GEOGRAPHICAL MEDICINE, 1976, 28 (04): : 355 - 358
12345