Measuring fecal steroids - Guidelines for practical application

被引:395
作者
Palme, R [1 ]
机构
[1] Univ Vet Med, Dept Nat Sci, Inst Biochem, A-1210 Vienna, Austria
来源
BIRD HORMONES AND BIRD MIGRATIONS: ANALYZING HORMONES IN DROPPINGS AND EGG YOLKS AND ASSESSING ADAPTATIONS IN LONG-DISTANCE MIGRATION | 2005年 / 1046卷
关键词
steroid hormones; estrogens; gestagens; androgens; glucocorticoids; noninvasive monitoring; feces/faeces; validation; sex differences; stress;
D O I
10.1196/annals.1343.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
During the past 20 years, measuring steroid hormone metabolites in fecal samples has become a widely appreciated technique, because it has proved to be a powerful, noninvasive tool that provides important information about an animal's endocrine status (adrenocortical activity and reproductive status). However, although sampling is relatively easy to perform and free of feedback, a careful consideration of various factors is necessary to achieve proper results that lead to sound conclusions. This article aims to provide guidelines for an adequate application of these techniques. It is meant as a checklist that addresses the main topics of concern, such as sample collection and storage, time delay extraction procedures, assay selection and validation, biological relevance, and some confounding factors. These issues are discussed briefly here and in more detail in other recent articles.
引用
收藏
页码:75 / 80
页数:6
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