Ultrasensitive detection of microcystin-LR with gold immunochromatographic assay assisted by a molecular imprinting technique

被引：40

作者：

Wu, Zhengzong ^{[1
]}

He, Deyun ^{[2
]}

Cui, Bo ^{[1
]}

Jin, Zhengyu ^{[3
]}

机构：

[1] Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Shandong, Peoples R China

[2] China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 283卷

关键词：

Microcystin-LR; Rapid analysis; Gold nanoparticles; Lateral flow; Molecular imprinting; RAPID DETECTION; SENSOR; IMMUNOSENSOR; APTASENSOR;

D O I：

10.1016/j.foodchem.2019.01.064

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A simple and highly sensitive lateral flow immunochromatographic assay (LFICA) towards microcystin-LR (MC-LR) was proposed in this study. Molecular imprinting technique was combined with enzyme assisted colorimetric method to substantially enhance the sensitivity of traditional LFICA. The target, i.e., MC-LR molecular, was first separated from the complex matrix by molecularly-imprinted polymers (MIPs). Then, the obtained MC-LR was detected by LFICA based on the blue color generated by an enzyme-substrate reaction. Using the proposed assay, under optimal conditions, the determination of MC-LR was doable in a wide linear range from 0.1 ng/mL to 100 ng/mL with a detection limit of 0.04 ng/mL. In addition, the reliability of the developed method was validated by determining MC-LR contents in real samples. The overall results suggested that the neoteric LFICA method possess great potential in sensitive detection of MC-LR.

引用

页码：517 / 521

页数：5

共 24 条

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