Neurogenesis of Adipose-derived Stem Cells in Hydrogel

被引：5

作者：

Xie, Xuewei ^{[1
,2
]}

Tang, Zhouping ^{[1
]}

Chen, Juan ^{[1
]}

Yang, Jie ^{[1
]}

Zeng, Wengao ^{[1
]}

Liu, Na ^{[1
]}

Liu, Yongming ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Univ, Tongji Hosp, Dept Neurol, Wuhan 430030, Peoples R China

[2] Second Mil Med Univ, Changzheng Hosp, Dept Neurol, Shanghai 200003, Peoples R China

来源：

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2011年 / 31卷 / 02期

关键词：

adipose-derived stem cells; hydrogel; neurogenesis; tissue engineering; STROMAL CELLS; TISSUE;

D O I：

10.1007/s11596-011-0246-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.

引用

页码：174 / 177

页数：4

共 17 条

[1] Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: Implications in soft tissue augmentation and reconstruction [J].