Hormonal regulation of proprotein convertase subtilisin/kexin type 5 expression during ovarian follicle development in the rat

被引：19

作者：

Bae, Jeong-A ^{[1
,2
]}

Park, Hyun-Jeong ^{[1
,2
]}

Seo, You-Mi ^{[1
,2
]}

Roh, Jaesook ^{[3
]}

Hsueh, Aaron J. W. ^{[4
]}

Chun, Sang-Young ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500712, South Korea

[2] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500712, South Korea

[3] Hanyang Univ, Med Ctr, Dept Obstet & Gynecol, Seoul 133792, South Korea

[4] Stanford Univ, Sch Med, Dept Obstet & Gynecol, Div Reprod Biol, Stanford, CA 94305 USA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2008年 / 289卷 / 1-2期

关键词：

proprotein convertase subtilisin/kexin type 5; ovary; follicle development; gonadotropin; ovulation;

D O I：

10.1016/j.mce.2008.04.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The proprotein convertase subtilisin/kexin (PCSKs), a family of subtilisin-like proteases, is the processing enzymes for the activation of many hormone precursors. The present study was designed to identify the PCSK isoform expressed in the ovary and to examine its expression in gonadotropin-stimulated rat ovary. Northern blot analysis of ovaries obtained from prepubertal rats revealed an increased expression of Pcsk5 messenger RNA (mRNA) during development with the highest levels at 21 days of age. Treatment of immature rats with PMSG further increased ovarian Pcsk5 expression, and in situ hybridization analysis revealed the localization of Pcsk5 mRNA in theca-interstitial cells of follicles in different sizes. Interestingly, treatment of PMSG-primed rats with hCG resulted in a transient stimulation of ovarian Pcsk5 mRNA levels within 3-6 h. In addition to theca-interstitial cells, hCG treatment induced the expression of Pcsk5 in granulosa cells of preovulatory follicles. Pcsk1, 2 and 4 mRNAs were not detected whereas Pcsk7 mRNA was slightly expressed. injection of a progestin antagonist RU486 or an inhibitor of 3 beta-hydroxysteroid dehydrogenase epostane at I h before hCG treatment inhibited hCG-induced Pcsk5 mRNA levels. Treatment with LH stimulated both Pcsk5 mRNA and protein levels in preovulatory follicles cultured in vitro. In addition, forskolin but not TPA stimulated Pcsk5 mRNA levels. RNase protection assay revealed that the soluble Pcsk5A variant was the predominant form stimulated by gonadotropins in the ovary. Finally, the predicted proprotein substrates cleaved by PCSK5 were analyzed in preovulatory follicles using regular expressions. The present study demonstrates PCSK5A as the gonadotropin-regulated PCSK isoform in the ovary, and its possible contribution to ovulation by processing pro-TGF beta and matrix metalloproteinase family. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：29 / 37

页数：9

共 58 条

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