DNA-modified graphene quantum dots as a sensing platform for detection of Hg2+ in living cells

被引：41

作者：

Zhao, Xin ^{[1
]}

Gao, Jinsuo ^{[1
]}

He, Xin ^{[1
]}

Cong, Longchao ^{[1
]}

Zhao, Huimin ^{[1
]}

Li, Xiaoyu ^{[2
]}

Tan, Feng ^{[1
]}

机构：

[1] Dalian Univ Technol, Sch Environm Sci & Technol, Key Lab Ind Ecol & Environm Engn MOE, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 49期

关键词：

GOLD NANOPARTICLES; SENSITIVE DETECTION; FLUORESCENT-PROBE; ELECTROCHEMICAL DETECTION; COLORIMETRIC DETECTION; CARBON NANOTUBES; ENERGY-TRANSFER; LEAD II; OXIDE; MERCURY;

D O I：

10.1039/c5ra06984j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Detection of metal ions in living cells is significant in environmental monitoring and health risk assessment. This paper reports a simple and facile method for the detection of Hg2+ ions in Hela cells through fluorescence imaging, which was based on the fluorescence quenching in thymine-rich DNA modified graphene quantum dots (DNA-GQDs) in the presence of Hg2+. The decrease in the fluorescence intensity was attributed to the electron transfer of DNA-GQDs due to Hg2+ bound to the thymine bases of the DNA, resulting in a T-T mismatch hairpin structure. The method shows high selectivity and sensitivity. This present sensing platform will have broad applications in biological imaging and environmental monitoring.

引用

页码：39587 / 39591

页数：5

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