Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica

被引：18

作者：

Cibrian-Jaramillo, Angelica ^{[1
,2
]}

Marler, Thomas E. ^{[3
]}

DeSalle, Rob ^{[2
]}

Brenner, Eric D. ^{[1
]}

机构：

[1] New York Bot Garden, Bronx, NY 10458 USA

[2] Amer Museum Nat Hist, Sackler Inst Comparat Genom, New York, NY 10024 USA

[3] Univ Guam, Coll Nat & Appl Sci, Western Pacific Trop Res Ctr, Mangilao, GU 96923 USA

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 04期

关键词：

cycad; Cycas micronesica; EST-STRS; invasives;

D O I：

10.1007/s10592-007-9447-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.

引用

页码：1051 / 1054

页数：4

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