Characterization of the antiproliferative activity of Xylopia aethiopica

被引：35

作者：

Choumessi, Aphrodite T. ^{[4
,5
]}

Danel, Mathieu ^{[1
,2
]}

Chassaing, Stefan ^{[1
,2
,6
]}

Truchet, Isabelle ^{[1
,2
]}

Penlap, Veronique B. ^{[4
]}

Pieme, Anatole Constant ^{[5
]}

Asonganyi, Tazoacha ^{[5
]}

Ducommun, Bernard ^{[1
,2
,3
,7
]}

Valette, Annie ^{[1
,2
]}

机构：

[1] Univ Toulouse, ITAV UMS3039, F-31106 Toulouse, France

[2] CNRS, ITAV UMS3039, F-31106 Toulouse, France

[3] CHU Toulouse, F-31059 Toulouse, France

[4] Univ Yaounde I, Fac Sci, Dept Biochem, Yaounde, Cameroon

[5] Univ Yaounde I, Fac Med & Biomed Sci, Dept Physiol Sci, Yaounde, Cameroon

[6] Univ Toulouse, LSPCMIB UMR5068, F-31062 Toulouse, France

[7] Ctr Pierre Potier, ITAV UMS3039, F-31106 Toulouse 1, France

来源：

CELL DIVISION | 2012年 / 7卷

关键词：

Xylopia aethiopica; ent-15-oxokaur-16-en-19-oic acid; cytotoxicity; DNA damage; ESSENTIAL OILS; ANTIMICROBIAL ACTIVITY; ANNONACEAE PROTECTS; MEDICINAL-PLANTS; FRUIT EXTRACT; ANTIOXIDANT;

D O I：

10.1186/1747-1028-7-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells. Results: We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative activity against a panel of cancer cell lines. The IC50 was estimated at 12 mu g/ml against HCT116 colon cancer cells, 7.5 mu g/ml and > 25 mu g/ml against U937 and KG1a leukemia cells, respectively. Upon fractionation of the extract by HPLC, the active fraction induced DNA damage, cell cycle arrest in G1 phase and apoptotic cell death. By using NMR and mass spectrometry, we determined the structure of the active natural product in the HPLC fraction as ent-15-oxokaur-16-en-19-oic acid. Conclusion: The main cytotoxic and DNA-damaging compound in ethanolic extracts of Xylopia aethiopica is ent-15-oxokaur-16-en-19-oic acid.

引用

页数：8

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