Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly

被引：355

作者：

Li, Hongjie ^{[1
,2
,3
]}

Janssens, Jasper ^{[4
,5
]}

De Waegeneer, Maxime ^{[4
,5
]}

Kolluru, Sai Saroja ^{[6
,7
,8
]}

Davie, Kristofer ^{[4
]}

Gardeux, Vincent ^{[9
,10
]}

Saelens, Wouter ^{[9
,10
]}

David, Fabrice ^{[9
,10
,11
]}

Brbic, Maria ^{[8
,12
]}

Leskovec, Jure ^{[4
,5
]}

McLaughlin, Colleen N. ^{[1
]}

Xie, Qijing ^{[1
]}

Jones, Robert C. ^{[6
,7
,8
]}

Brueckner, Katja ^{[13
]}

Shim, Jiwon ^{[14
]}

Tattikota, Sudhir Gopal ^{[15
,16
]}

Schnorrer, Frank ^{[17
]}

Rust, Katja ^{[18
,19
]}

Nystul, Todd G. ^{[19
]}

Carvalho-Santos, Zita ^{[20
]}

Ribeiro, Carlos ^{[20
]}

Pal, Soumitra ^{[21
]}

Mahadevaraju, Sharvani ^{[22
]}

Przytycka, Teresa M. ^{[21
]}

Allen, Aaron M. ^{[23
]}

Goodwin, Stephen F. ^{[23
]}

Berry, Cameron W. ^{[24
]}

Fuller, Margaret T. ^{[24
]}

White-Cooper, Helen ^{[25
]}

Matunis, Erika L. ^{[26
]}

DiNardo, Stephen ^{[27
,28
]}

Galenza, Anthony ^{[29
]}

O'Brien, Lucy Erin ^{[29
]}

Dow, Julian A. T. ^{[30
]}

Jasper, Heinrich ^{[31
]}

Oliver, Brian ^{[22
]}

Perrimon, Norbert ^{[15
,16
]}

Deplancke, Bart ^{[9
,10
]}

Quake, Stephen R. ^{[6
,7
,8
]}

Luo, Liqun ^{[1
]}

Aerts, Stein ^{[4
,5
]}

机构：

[1] Stanford Univ, Howard Hughes Med Inst, Dept Biol, Stanford, CA 94305 USA

[2] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA

[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[4] Katholieke Univ Leuven, VIB KU Leuven Ctr Brain & Dis Res, B-3000 Leuven, Belgium

[5] Katholieke Univ Leuven, Lab Computat Biol, Dept Human Genet, B-3000 Leuven, Belgium

[6] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[7] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[8] Chan Zuckerberg Biohub, San Francisco, CA 94158 USA

[9] Ecole Polytech Fed Lausanne EPFL, Sch Life Sci, Inst Bioengn, Lab Syst Biol & Genet, CH-1015 Lausanne, Switzerland

[10] Swiss Inst Bioinformat, CH-1015 Lausanne, Switzerland

[11] Ecole Polytech Fed Lausanne, Bioinformat Competence Ctr, Lausanne, Switzerland

[12] Stanford Univ, Dept Comp Sci, Stanford, CA 94305 USA

[13] Univ Calif San Francisco, Dept Cell & Tissue Biol, San Francisco, CA 94143 USA

[14] Hanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea

[15] Harvard Med Sch, Dept Genet, Blavatnik Inst, Boston, MA 02115 USA

[16] Harvard Med Sch, Howard Hughes Med Inst, Boston, MA 02115 USA

[17] Aix Marseille Univ, Turing Ctr Living Syst, CNRS, IBDM UMR 7288, F-13009 Marseille, France

[18] Philipps Univ, Inst Physiol & Pathophysiol, Dept Mol Cell Physiol, Marburg, Germany

[19] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA

[20] Champalimaud Ctr Unknown, Champalimaud Res, Behav & Metab Lab, Lisbon, Portugal

[21] NLM, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA

[22] NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD 20892 USA

[23] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 3SR, England

[24] Stanford Univ, Dept Dev Biol & Genet, Sch Med, Stanford, CA 94305 USA

[25] Cardiff Univ, Mol Biosci Div, Cardiff CF10 3AX, S Glam, Wales

[26] Johns Hopkins Univ, Dept Cell Biol, Sch Med, Baltimore, MD 21205 USA

[27] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA

[28] Penn Inst Regenerat Med, Philadelphia, PA 19104 USA

[29] Stanford Univ, Dept Mol & Cellular Physiol, Sch Med, Stanford, CA 94305 USA

[30] Univ Glasgow, Coll Med Vet & Life Sci, Inst Mol Cell & Syst Biol, Glasgow G12 8QQ, Lanark, Scotland

[31] Genentech Inc, Immunol Discovery, San Francisco, CA 94080 USA

来源：

SCIENCE | 2022年 / 375卷 / 6584期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DROSOPHILA; RESOURCE; SEX; EVOLUTION;

D O I：

10.1126/science.abk2432

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae, that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type-related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution.

引用

页码：991 / +

页数：84

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