Characterization of a novel lipid A structure isolated from Azospirillum lipoferum lipopolysaccharide

被引：19

作者：

Choma, Adam ^{[1
]}

Komaniecka, Iwona ^{[1
]}

机构：

[1] Marie Curie Sklodowska Univ, Dept Gen Microbiol, PL-20033 Lublin, Poland

来源：

CARBOHYDRATE RESEARCH | 2008年 / 343卷 / 04期

关键词：

Azospirillum lipoferum; lipid a structure; glucosamine;

D O I：

10.1016/j.carres.2008.01.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of lipid A from Azospirillum lipoferum, a plant-growth-promoting rhizobacterium, was investigated. It was determined by chemical analysis, mass spectrometric methods, as well as 1D and 2D NMR spectroscopy. Because of the presence of substituents, the investigated lipid A differs from typical enterobacterial lipid A molecules. Its backbone is composed of a beta-(1,6)linked D-glucosamine disaccharide but lacks phosphate residues. Moreover, the reducing end of the backbone (position C-1) is substituted with alpha-linked D-galacturonic acid. 3-hydroxypalmitoyl residues are exclusively connected to amino groups of the glucosamine disaccharide. Hydroxyls at positions C-3 and C-3' are esterified with 3-hydroxymyristic acids. Primary polar fatty acids are partially substituted by nonpolar fatty acids (namely, 18:0, 18:1 or 16:0), forming acyloxyacyl moieties. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：799 / 804

页数：6

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