Costs of resistance: A test using transgenic Arabidopsis thaliana

被引:86
作者
Bergelson, J
Purrington, CB
Palm, CJ
LopezGutierrez, JC
机构
[1] Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637
关键词
D O I
10.1098/rspb.1996.0242
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Evolutionary biologists have long attributed polymorphisms in resistance status to fitness costs of resistance traits. Nevertheless, pleiotropic fitness costs of resistance have been notoriously difficult to detect. We have transformed Arabidopsis thaliana with a mutant acetolactate synthase gene that confers resistance to the herbicide, chlorsulfuron. Our experiment revealed a 34% reduction in the lifetime seed production of transgenic, herbicide resistant Arabidopsis thaliana relative to their susceptible null-segregants. Our experimental design allows us to conclude that this fitness cost of resistance is caused by the pleiotropic effect of the introduced acetolactate synthase gene rather than other potential costs associated with the plasmid or mutational changes induced by plant transformation. In addition, we can attribute the cost of resistance to the presence of the resistance gene rather than an increase in gene dosage. The implications of these results for the risk assessment of transgenic crops are discussed.
引用
收藏
页码:1659 / 1663
页数:5
相关论文
共 33 条
[1]   THE COST OF DEFENSE AGAINST HERBIVORES - AN EXPERIMENTAL-STUDY OF TRICHOME PRODUCTION IN BRASSICA-RAPA [J].
AGREN, J ;
SCHEMSKE, DW .
AMERICAN NATURALIST, 1993, 141 (02) :338-350
[2]   Surveying patterns in the cost of resistance in plants [J].
Bergelson, J ;
Purrington, CB .
AMERICAN NATURALIST, 1996, 148 (03) :536-558
[3]   BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].
BEVAN, M .
NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721
[4]   ACETOLACTATE SYNTHASE IS THE SITE OF ACTION OF 2 SULFONYLUREA HERBICIDES IN HIGHER-PLANTS [J].
CHALEFF, RS ;
MAUVAIS, CJ .
SCIENCE, 1984, 224 (4656) :1443-1445
[5]  
COCCIOLONE SM, 1993, GENETICS, V135, P575
[6]   RESOURCE AVAILABILITY AND PLANT ANTIHERBIVORE DEFENSE [J].
COLEY, PD ;
BRYANT, JP ;
CHAPIN, FS .
SCIENCE, 1985, 230 (4728) :895-899
[7]   ECOLOGY OF TRANSGENIC OILSEED RAPE IN NATURAL HABITATS [J].
CRAWLEY, MJ ;
HAILS, RS ;
REES, M ;
KOHN, D ;
BUXTON, J .
NATURE, 1993, 363 (6430) :620-623
[8]   INHIBITION OF ACETOLACTATE SYNTHASE IN SUSCEPTIBLE AND RESISTANT BIOTYPES OF STELLARIA-MEDIA [J].
DEVINE, MD ;
MARLES, MAS ;
HALL, LM .
PESTICIDE SCIENCE, 1991, 31 (03) :273-280
[9]   RAPID GERMINATION OF SULFONYLUREA-RESISTANT KOCHIA-SCOPARIA L ACCESSIONS IS ASSOCIATED WITH ELEVATED SEED LEVELS OF BRANCHED-CHAIN AMINO-ACIDS [J].
DYER, WE ;
CHEE, PW ;
FAY, PK .
WEED SCIENCE, 1993, 41 (01) :18-22
[10]   PHYSIOLOGICAL-RESPONSE TO MOISTURE SUPPLY OF CYANOGENIC AND ACYANOGENIC PHENOTYPES OF TRIFOLIUM-REPENS-L AND LOTUS-CORNICULATUS L [J].
FOULDS, W .
HEREDITY, 1977, 39 (OCT) :219-234