Viridibacillus soli sp. nov., isolated from forest soil in Ailaoshan National Nature Reserve

A Gram stain-positive, rod-shaped, and subterminal endospore-forming bacterium, designated strain YIM B01967(T), was isolated from a forest soil sample collected in Ailaoshan National Nature Reserve, Yuxi City, Xinpin county, Yunnan province, China. Strain YIM B01967(T) showed the highest 16S rRNA gene sequence similarity with Viridibacillus arvi (99.1%) and Viridibacillus arenosi (98.9%). Based on the phylogenetic and 16S rRNA gene sequence results, strain YIM B01967(T) was affiliated to the genus Viridibacillus. The growth of YIM B01967(T) was observed at 15-35 degrees C (optimum, 28 degrees C), pH 7.0-9.0 (optimum, pH 7.5) and in the presence of 0-2% (w/v) NaCl (optimum in 2% NaCl). The cell wall sugars include ribose, glucose, arabinose, galactose, and mannose. The quinone system consisted of the major compound MK-8 and moderate amounts of MK-7. The major fatty acids (> 10%) included iso-C-15:0, anteiso-C-15:0, C-16:1 omega 10c. The major polar lipids profile included DPG, PME. The cell wall peptidoglycan was most likely of the type A4 alpha with an l-Lys-d-Asp interpeptide bridge. The genomic DNA G + C content of strain YIM B01967(T) was 36.3 mol%. The ANI and digital DNA-DNA hybridization (dDDH) values between strain YIM B01967(T) and Viridibacillus arvi DSM 16317( T), Viridibacillus arenosi DSM 16319( T) were 61.0% and 32.1%, 60.0% and 33.1% based on the draft genome sequence. The results support the conclusion that strain YIM B01967(T) represents a novel species of the genus Viridibacillus, for which the name Viridibacillus soli sp. nov., is proposed. The type strain is YIM B01967(T) (= KCTC 43249( T) = CGMCC 1.18436( T)).