Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

被引：6

作者：

Gahtory, Digvijay ^{[1
]}

Murtola, Merita ^{[2
]}

Smulders, Maarten M. J. ^{[1
]}

Wennekes, Tom ^{[1
,3
,4
]}

Zuilhof, Han ^{[1
,5
,6
]}

Stromberg, Roger ^{[2
]}

Albada, Bauke ^{[1
]}

机构：

[1] Wageningen Univ & Res, Lab Organ Chem, Stippeneng 4, NL-6708 WE Wageningen, Netherlands

[2] Dept Biosci & Nutr, Halsovagen 7, S-14183 Huddinge, Sweden

[3] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Chem Biol & Drug Discovery, Utrecht, Netherlands

[4] Univ Utrecht, Bijvoet Ctr Biomol Res, Utrecht, Netherlands

[5] King Abdulaziz Univ, Dept Chem & Mat Engn, Jeddah, Saudi Arabia

[6] Tianjin Univ, Sch Pharmaceut Sci & Technol, 92 Weijin Rd, Tianjin 300072, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 32期

关键词：

THIAZOLE ORANGE; FLUORESCENT BASE; FIT PROBES; HYBRIDIZATION; DNA; BACKBONE; DELIVERY; PNAZYMES;

D O I：

10.1039/c7ob01592e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-D-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.

引用

页码：6710 / 6714

页数：5

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