Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde

被引：35

作者：

Dousa, Michal ^{[1
]}

Brichac, Jiri ^{[1
]}

Gibala, Petr ^{[1
]}

Lehnert, Petr ^{[1
,2
]}

机构：

[1] As Praha, Zentiva, Prague 10237 10, Czech Republic

[2] Palacky Univ, Dept Analyt Chem, Olomouc 77146, Czech Republic

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2011年 / 54卷 / 05期

关键词：

HILIC; Lysine; Arginine; Postcolumn derivatization; PERFORMANCE LIQUID-CHROMATOGRAPHY; FREE AMINO-ACIDS; IBUPROFEN LYSINATE; PEPTIDES; SEPARATION; RETENTION; COLUMN; BIOAVAILABILITY; CEPHALEXIN; SALT;

D O I：

10.1016/j.jpba.2010.11.026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic). (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：972 / 978

页数：7

共 36 条

[1] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].