RAPD-PCR characterisation of two Enterococcus lactis strains and their potential on Listeria monocytogenes growth behaviour in stored chicken breast meats: Generalised linear mixed-effects approaches

被引：13

作者：

Ben Braiek, Olfa ^{[1
,2
]}

Smaoui, Slim ^{[3
]}

Ennouri, Karim ^{[3
]}

Morandi, Stefano ^{[4
]}

Cremonesi, Paola ^{[5
]}

Hani, Khaled ^{[6
]}

Ghrairi, Taoufik ^{[1
,2
]}

机构：

[1] Univ Tunis El Manar, Fac Sci Tunis, Lab Microorganisms & Act Biomol LMBA, Tunis, Tunisia

[2] ISSTE, RLEST, Technopole Borj Cedria, Tunis, Tunisia

[3] Ctr Biotechnol Sfax, Lab Microorganisms & Biomol, Sfax, Tunisia

[4] CNR ISPA, Italian Natl Res Council, Inst Sci Food Prod, Milan, Italy

[5] CNR ISPA, Italian Natl Res Council, Inst Agr Biol & Biotechnol, Lodi, Italy

[6] Fac Med Ibn El Jazzar Sousse, Dept Biochem, UR012 ES03, Sousse, Italy

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2019年 / 99卷

关键词：

Enterococcus lactic; Listeria monocytogenes; Chicken breast meat; Generalised linear mixed-effects models; Food safety; MOLECULAR CHARACTERIZATION; SAFETY CHARACTERIZATION; PROBIOTIC PROPERTIES; FERMENTED SAUSAGES; FOOD SAFETY; FAECIUM; BACTERIOCINS; RAW; INHIBITION; PATHOGENS;

D O I：

10.1016/j.lwt.2018.09.053

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study deals with the genetic characterisation of two enterocin-producing Enterococcus lactis strains named Q1 and 4CP3 using Randomly Amplified Polymorphic DNA (RAPD) PCR and the effect of their separate additions in the control of Listeria monocytogenes in refrigerated chicken breast meat. These strains demonstrated interesting in vitro antimicrobial activity towards L. monocytogenes EGDe 107776 by agar assay. To evaluate the in situ effect of E. lcids strains against L. monocytogenes in white meat model, chicken breast were artificially contaminated with 10(5) CFU/g of L. monocytogenes and inoculated with E. lactis Q1 and 4CP3 of each at 10(7) CFU/g. The pathogen counts were monitored during cold storage for 28 days. The anti-listerial effect of the enterococcal cultures was examined using linear (ANOVA) and general (ANCOVA) models and it has been shown that both of them controlled L monocytogenes by decreasing (P < 0.05) and suppressing the pathogen growth in refrigerated chicken meats during 28 days of storage. The present study shows that our E. lactic strains have great potential as protective cultures and might be used for preventing the growth of pathogenic bacteria such as L. monocytogenes in meat products during their refrigerated storage.

引用

页码：244 / 253

页数：10

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