Flow-injection with enhanced evaporative light scattering detector detection and quantification of human serum albumin using gold nanoparticles

被引：8

作者：

Wang, Tao ^{[1
,2
]}

Chen, Mengbin ^{[1
]}

Niu, Caixia ^{[1
]}

Liu, Ning ^{[3
]}

Wang, Le ^{[1
]}

Sun, Binjie ^{[1
]}

Hua, Wenhao ^{[4
]}

Ouyang, Jin ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Key Lab Theoret & Computat Photochem, Minist Educ, Beijing 100875, Peoples R China

[2] Changzhi Med Coll, Dept Pharm, Changzhi 046000, Peoples R China

[3] China Pharmaceut Univ, Nanjing 210009, Jiangsu, Peoples R China

[4] Capital Med Univ, Beijing Ditan Hosp, Dept Clin Lab, Beijing 100050, Peoples R China

来源：

ANALYTICAL METHODS | 2015年 / 7卷 / 07期

关键词：

LIQUID-CHROMATOGRAPHY; PROTEINS; PROBE; AGGREGATION; ADSORPTION; SURFACES; BINDING; ASSAY; SIZE; HPLC;

D O I：

10.1039/c4ay02918f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method, based on evaporative light scattering detection (ELSD), enhanced by gold nanoparticles (GNPs), was established to determine human serum albumin (HSA). In our case, under the optimal conditions, the calibration interval was 43.0-2.50 x 10(4) ng with a detection limit of 21.0 ng for HSA. The analysis speed of ELSD is fast: it takes only 2 min to analyze each sample. We also observed acceptable agreement between the new method and the BCG method. The new method was proved to be simple, rapid and sensitive for the determination of HSA. Our results demonstrate that ELSD combined with GNPs may have great potential value for clinical trials which will meet the needs of clinical rapid analysis.

引用

页码：3185 / 3192

页数：8

共 35 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] Probing BSA binding to citrate-coated gold nanoparticles and surfaces [J].

Brewer, SH ;

Glomm, WR ;

Johnson, MC ;

Knag, MK ;

Franzen, S .

LANGMUIR, 2005, 21 (20) :9303-9307

[3] EVAPORATIVE ANALYZER AS A MASS DETECTOR FOR LIQUID-CHROMATOGRAPHY [J].

CHARLESWORTH, JM .

ANALYTICAL CHEMISTRY, 1978, 50 (11) :1414-1420

[4] Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells [J].

Chithrani, BD ;

Ghazani, AA ;

Chan, WCW .

NANO LETTERS, 2006, 6 (04) :662-668

[5] Albumin standards and the measurement of serum albumin with bromcresol green [J].

Doumas, BT ;

Watson, WA ;

Biggs, HG .

CLINICA CHIMICA ACTA, 1997, 258 (01) :21-30

[6]

FORD DL, 1966, J OIL COLOUR CHEM AS, V49, P299

[7] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[8] 177Lu-DO3A-HSA-ZEGFR:1907: characterization as a potential radiopharmaceutical for radionuclide therapy of EGFR-expressing head and neck carcinomas [J].

Hoppmann, Susan ;

Qi, Shibo ;

Miao, Zheng ;

Liu, Hongguang ;

Jiang, Han ;

Cutler, Cathy S. ;

Bao, Ande ;

Cheng, Zhen .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2012, 17 (05) :709-718

[9] Quantification and identification of bioactive metabolites from Kalopanacis Cortex by HPLC with evaporative light scattering detection and ESI quadrupole TOF MS [J].

Liao, Lijuan ;

Won, Tae Hyung ;

Kim, Young Ho ;

Shin, Jongheon .

JOURNAL OF SEPARATION SCIENCE, 2014, 37 (05) :505-514

[10] A gold nanorod based colorimetric probe for the rapid and selective detection of Cu2+ ions [J].

Liu, Jing-Min ;

Wang, He-Fang ;

Yan, Xiu-Ping .

ANALYST, 2011, 136 (19) :3904-3910

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