Anti-bacterial and anti-microbial aging effects of resin-based sealant modified by quaternary ammonium monomers

Objectives: Pit and fissure sealant is used in the prevention of dental caries. However, commercial pit and fissure sealant lacks persistent antibacterial properties. Dimethylaminododecyl methacrylate (DMADDM) was added to pit and fissure sealants to give it sustainable antibacterial properties and anti-microbial aging properties. Methods: Resin-based sealant was used as a control. Novel sealants were made with DMADDM. Atomic force microscope observation, curing depth, cytotoxicity, lactic acid measurement, hardness and microleakage were measured. Saliva-derived biofilms were grown on sealants. Biofilm metabolic activity, lactic acid production and biomass accumulation were measured. Results: Incorporating DMADDM did not increase the cytotoxicity or change the physical properties when the mass fraction of the DMADDM was 2.5-10%. The modification decreased the amount of bacterial biofilm, metabolic activity, lactic acid production and exopolysaccharide (EPS) in the saliva biofilms. It also provided anti-microbial aging properties. Conclusion: The incorporation of DMADDM improved the antibacterial and anti-microbial aging effects of the material. It demonstrated a sustained antibacterial effect. The antibacterial and anti-microbial aging modification might be a potential choice for future clinical applications to inhibit dental caries, especially for children at high caries risk. Clinical Significance: The antibacterial and anti-microbial aging modification might be a potential choice for future clinical applications to prevent dental caries, especially for individuals at high caries risk.