Drought stress tolerance is seen in almost all plants but its extent varies from species to species and even within species Drought stress produced changes in photosynthetic pigments and components One of the most common stress tolerance strategies is Increase in organic solutes production such as prolin This experiment was conducted to evaluate the effect of drought stress on pigments, prolin and seed protein of wheat and triticale in Damghan, Iran Experimental design was split plot based on randomized complete block with three replications Four irrigation levels (control and no irrigation from stem elongation-S, booting-B and milky-M stages to harvest) were considered as main plot and two cereal species (wheat and triticale) were considered as sub plot Drought stress, especially in S treatment, declined chlorophyll a and b and carotene, but this treatment had the highest leaf prolin level and seed protein percent Triticale had more content of chlorophyll b, carotene and seed protein There was not any significant different between chlorophyll a and prolin of wheat and triticale Totally, the result show in drought stress condition triticale with more yield than wheat had high quantity of chlorophyll a and b, carotene and prolin