N-terminal sequence of amino acids and some properties of an acid-stable alpha-amylase from citric acid koji (Aspergillus usanii var)

被引：7
作者：
Suganuma, T [1 ]
Tahara, N [1 ]
Kitahara, K [1 ]
Nagahama, T [1 ]
Inuzuka, K [1 ]
机构：
[1] KYUSHU KAKO CO, KAGOSHIMA 893, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 01期
关键词：
acid-stable alpha-amylase; N-terminal sequence; Aspergillus usamii; citric acid koji; maltopentaose;
D O I：
10.1271/bbb.60.177
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An acid-stable alpha-amylase (AA) was purified from an acidic extract of citric acid-koji (A. usamii var.). The N-terminal sequence of the first 20 amino acids of the enzyme was identical with that of AA from A. niger, but the two enzymes differed in molecular weight, HPLC analysis for identifying the anomers of products indicated that the AA hydrolyzed maltopentaose (G5) at the third glycoside bond predominantly, which differed from Taka-amylase A and the neutral alpha-amylase (NA) from the citric acid-koji.
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收藏
页码：177 / 179
页数：3
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