Amperometric glucose sensor based on the glucose oxidase enzyme immobilized on graphite rod electrode modified with Fe3O4-CS-Au magnetic nanoparticles

被引：20

作者：

Onay, Aykut ^{[1
]}

Dogan, Uzeyir ^{[1
]}

Ciftci, Hakan ^{[2
]}

Cetin, Demet ^{[3
]}

Suludere, Zekiye ^{[4
]}

Tamer, Ugur ^{[1
]}

机构：

[1] Gazi Univ, Dept Analyt Chem, Fac Pharm, TR-06330 Ankara, Turkey

[2] Kirikkale Univ, Kirikkale Vocat High Sch, Dept Chem & Chem Proc Technol, TR-71450 Yahsihan, Kirikkale, Turkey

[3] Gazi Univ, Gazi Fac Educ, Dept Math & Sci Educ, TR-06500 Ankara, Turkey

[4] Gazi Univ, Dept Biol, Fac Sci, TR-06500 Ankara, Turkey

来源：

IONICS | 2018年 / 24卷 / 12期

关键词：

Glucose; Electrochemical sensor; Magnetic nanoparticle; Graphite rod; Chitosan; REDUCED GRAPHENE OXIDE; GLASSY-CARBON ELECTRODE; GOLD NANOPARTICLES; SENSITIVE DETERMINATION; DIRECT ELECTROCHEMISTRY; CHITOSAN; SILVER; ACID; NANOCOMPOSITE; SPECTROSCOPY;

D O I：

10.1007/s11581-018-2559-6

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this report, an amperometric detection method for blood glucose level was developed benefiting from glucose oxidase (GO) enzyme immobilization on nanoparticle modified graphite rod (GR) electrode. Fe3O4-CS-Au magnetic nanoparticles were synthesized, characterized by TEM, UV-Vis, magnetometry, FTIR, and zeta potential measurements and used for the modification of GR electrode. This modified electrode was used for the detection of glucose level amperometrically at 0.6 V. The obtained calibration graph was linear in the range of 5-30mM glucose concentration with a coefficient of determination (R-2) 0.9971. The limit of detection (LOD) and limit of quantification (LOQ) values were calculated as 0.55 and 1.83mM, respectively. The modified GR electrode showed excellent selectivity in the presence of dopamine, ascorbic acid, and uric acid. The applicability of the developed method was examined in real blood samples by comparing the results obtained from commercial glucose sensor. This novel glucose detection method exhibited fast amperometric response, long storage time, and good selectivity.

引用

页码：4015 / 4022

页数：8

共 36 条

[11] Nanoparticle embedded chitosan film for agglomeration free TEM images [J].

Dogan, Uzeyir ;

Ciftci, Hakan ;

Cetin, Demet ;

Suludere, Zekiye ;

Tamer, Ugur .

MICROSCOPY RESEARCH AND TECHNIQUE, 2017, 80 (02) :163-166

[12] Rapid detection of bacteria based on homogenous immunoassay using chitosan modified quantum dots [J].

Dogan, Uzeyir ;

Kasap, Esin ;

Cetin, Demet ;

Suludere, Zekiye ;

Boyaci, Ismail Hakki ;

Turkyilmaz, Canan ;

Ertas, Nusret ;

Tamer, Ugur .

SENSORS AND ACTUATORS B-CHEMICAL, 2016, 233 :369-378

[13] Gold nanoparticle embedded, self-sustained chitosan films as substrates for surface-enhanced Raman scattering [J].

dos Santos, DS ;

Goulet, PJG ;

Pieczonka, NPW ;

Oliveira, ON ;

Aroca, RF .

LANGMUIR, 2004, 20 (23) :10273-10277

[14] Extinction, emission, and scattering spectroscopy of 5-50 nm citrate-coated gold nanoparticles: An argument for curvature effects on aggregation [J].

Esfahani, Milad Rabbani ;

Pallem, Vasanta L. ;

Stretz, Holly A. ;

Wells, Martha J. M. .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2017, 175 :100-109

[15] Direct electrochemistry of glucose oxidase immobilized on Au nanoparticles-functionalized 3D hierarchically ZnO nanostructures and its application to bioelectrochemical glucose sensor [J].

Fang, Linxia ;

Liu, Bing ;

Liu, Lulu ;

Li, Yuehua ;

Huang, Kejing ;

Zhang, Qiuyu .

SENSORS AND ACTUATORS B-CHEMICAL, 2016, 222 :1096-1102

[16] Simple one-pot preparation of chitosan-reduced graphene oxide-Au nanoparticles hybrids for glucose sensing [J].

Fang, Yuxin ;

Zhang, Di ;

Guo, Yi ;

Guo, Yongming ;

Chen, Qiang .

SENSORS AND ACTUATORS B-CHEMICAL, 2015, 221 :265-272

[17] Glucose biosensor based on glucose oxidase and gold nanoparticles of different sizes covered by polypyrrole layer [J].

German, Natalija ;

Ramanavicius, Arunas ;

Voronovic, Jaroslav ;

Ramanaviciene, Almira .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2012, 413 :224-230

[18] Chitosan mediated assembly of gold nanoparticles multilayer [J].

Huang, HZ ;

Yang, XR .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2003, 226 (1-3) :77-86

[19] Silver nanoparticles-β-cyclodextrin-graphene nanocomposites based biosensor for guanine and adenine sensing [J].

Hui, Yuchen ;

Ma, Xiaoyan ;

Hou, Xiuzhang ;

Chen, Fang ;

Yu, Jie .

IONICS, 2015, 21 (06) :1751-1759

[20] Green synthesis of silver nanoparticle-decorated porous reduced graphene oxide for antibacterial non-enzymatic glucose sensors [J].

Le Thuy Hoa ;

Ngo Thi Yen Linh ;

Chung, Jin Suk ;

Hur, Seung Hyun .

IONICS, 2017, 23 (06) :1525-1532

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