Preparation, optimization, and characterization of chitosan-sepiolite nanocomposite films for wound healing

In this study, a series of chitosan-sepiolite (CS-SEP) nanocomposites films were prepared by using a conventional solution casting method. The effect of sepiolite on physicochemical and biological properties of the prepared nanocomposite films was studied by various techniques such as Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and x-ray diffraction (XRD) to name a few. In WCA measurements, the decrease of contact angle from 78.51 degrees (CS) to 71.29 degrees (CS7SEP3) reaffirms the water holding nature of sepiolite, which enables to create moist environment essentially required for wound healing. Further, addition of sepiolite tremendously increased WVTR, folding endurance, porosity, and blood clotting ability of the prepared nanocomposites. Furthermore, CS-SEP nanocomposite films exhibit better antibacterial activity than that of chitosan against gram positive (B. subtilis) and gram negative bacteria (E. coli). Moreover, the percentage of hemolysis and degradation study indicated that the prepared nanocomposite films were non-hemolytic in nature and decomposed nearly 40% in four weeks. In addition, cytotoxicity assay showed that the prepared nanocomposite film i.e. CS7SEP3 exhibited better cell viability and cell proliferation rate against L929 mouse fibroblast cells as compared to CS and hence, the prepared nanocomposite film can be used as a promising candidate for wound management.