Depth- and strain-dependent mechanical and electromechanical properties of full-thickness bovine articular cartilage in confined compression

被引:222
作者
Chen, AC [1 ]
Bae, WC [1 ]
Schinagl, RM [1 ]
Sah, RL [1 ]
机构
[1] Univ Calif San Diego, Inst Biomed Engn, Dept Bioengn, La Jolla, CA 92093 USA
基金
美国国家航空航天局; 美国国家科学基金会;
关键词
cartilage; permeability; electrokinetic coefficient; inhomogeneous; strain-dependent;
D O I
10.1016/S0021-9290(00)00170-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Compression tests have often been performed to assess the biomechanical properties of full-thickness articular cartilage. We tested whether the apparent homogeneous strain-dependent properties, deduced from such tests, reflect both strain- and depth-dependent material properties. Full-thickness bovine articular cartilage was tested by oscillatory confined compression superimposed on a static offset up to 45%, and the data fit to estimate modulus, permeability, and electrokinetic coefficient assuming homogeneity. Additional tests on partial-thickness cartilage were then performed to assess depth- and strain-dependent properties in an inhomogeneous model, assuming three discrete layers (i = 1 starting from the articular surface, to i = 3 up to the subchondral bone). Estimates of the zero-strain equilibrium confined compression modulus (H-A0), the zero-strain permeability (k(p0)) and deformation dependence constant (M), and the deformation-depondent electrokinetic coefficient (k(e)) differed among individual layers of cartilage and full-thickness cartilage. H-A0(i) increased From layer 1 to 3 (0.27 to 0.71MPa), and bracketed the apparent homogeneous value (0.47 MPa).k(p0)(i) decreased from layer 1 to 3 (4.6 x 10-(15) to 0.50 x 10(-15) m/Pa s) and was less than the homogeneous value (7.3 x 10(-15) m(2)/Pa s), while M-I increased from layer 1 to 3 (5.5 to 7.4) and became similar to the homogeneous value (8.4). The amplitude of k(e)(i) increased markedly with compressive strain, as did the homogeneous value; at low strain, it was lowest near the articular surface and increased to a peak in the middle-deep region. These results help to interpret the biomechanical assessment of full-thickness articular cartilage. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1 / 12
页数:12
相关论文
共 42 条
[1]   VARIATIONS IN THE INTRINSIC MECHANICAL PROTERTIES OF HUMAN ARTICULAR-CARTILAGE WITH AGE, DEGENERATION, AND WATER-CONTENT [J].
ARMSTRONG, CG ;
MOW, VC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1982, 64 (01) :88-94
[2]   Finite deformation biphasic material properties of bovine articular cartilage from confined compression experiments [J].
Ateshian, GA ;
Warden, WH ;
Kim, JJ ;
Grelsamer, RP ;
Mow, VC .
JOURNAL OF BIOMECHANICS, 1997, 30 (11-12) :1157-1164
[3]   NONDESTRUCTIVE DETECTION OF CARTILAGE DEGENERATION USING ELECTROMECHANICAL SURFACE SPECTROSCOPY [J].
BERKENBLIT, SI ;
FRANK, EH ;
SALANT, EP ;
GRODZINSKY, AJ .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1994, 116 (04) :384-392
[4]   Activation and inhibition of endogenous matrix metalloproteinases in articular cartilage: Effects on composition and biophysical properties [J].
Bonassar, LJ ;
Stinn, JL ;
Paguio, CG ;
Frank, EH ;
Moore, VL ;
Lark, MW ;
Sandy, JD ;
Hollander, AP ;
Poole, AR ;
Grodzinsky, AJ .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 333 (02) :359-367
[5]  
Buschmann MD, 1998, J BIOMECH, V31, P171, DOI 10.1016/S0021-9290(97)00124-3
[6]   CHONDROCYTES IN AGAROSE CULTURE SYNTHESIZE A MECHANICALLY FUNCTIONAL EXTRACELLULAR-MATRIX [J].
BUSCHMANN, MD ;
GLUZBAND, YA ;
GRODZINSKY, AJ ;
KIMURA, JH ;
HUNZIKER, EB .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1992, 10 (06) :745-758
[7]   Streaming potentials during the confined compression creep test of normal and proteoglycan-depleted cartilage [J].
Chen, AC ;
Nguyen, TT ;
Sah, RL .
ANNALS OF BIOMEDICAL ENGINEERING, 1997, 25 (02) :269-277
[8]   Mature full-thickness articular cartilage explants attached to bone are physiologically stable over long-term culture in serum-free media [J].
Dumont, J ;
Ionescu, M ;
Reiner, A ;
Poole, AR ;
Tran-Khanh, N ;
Hoemann, CD ;
McKee, MD ;
Buschmann, MD .
CONNECTIVE TISSUE RESEARCH, 1999, 40 (04) :259-+
[9]  
EISENBERG SR, 1988, PHYSICOCHEM HYDRODYN, V10, P517
[10]   IMPROVED QUANTITATION AND DISCRIMINATION OF SULFATED GLYCOSAMINOGLYCANS BY USE OF DIMETHYLMETHYLENE BLUE [J].
FARNDALE, RW ;
BUTTLE, DJ ;
BARRETT, AJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 883 (02) :173-177