PHYLLODULCIN PROTECTS PC12 CELLS AGAINST THE INJURY INDUCED BY OXYGEN AND GLUCOSE DEPRIVATION-RESTORATION

Phyllodulcin is a natural coumarin derivative found in Hydrangea macrophylla. To discover effective therapeutics for cerebral ischemia-reperfusion injury, we investigated the protective effects of phyllodulcin on PC12 cells injury induced by oxygenation and glucose deprivation/restoration (OGD/R). As a result, phyllodulcin can improve the cell viability and attenuate the extracellular lactate dehydrogenase release. Meanwhile, phyllodulcin can ameliorate the dysfunction of mitochondria by reducing reactive oxygen species production. decreasing the intracellular calcium level and increasing the mitochondrial membrane potential. ROS-associaled OGD/R can induce cell apoptosis through the mitochondrial pathway. Phyllodulcin can inhibit apoptosis of PC12 cells through down-regulation of Caspase-3 and flax as well as up-regulation of Bcl-2. These results indicate phyllodukin can protect PC12 cells against the damage induced by OGD/R. This investigation gives promising evidences for the therapy of cerebral ischemia-reperfusion injury.