Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01

被引：84
作者：
Liu, Chu-Ting [1 ]
Chu, Fang-Jung [1 ]
Chou, Cheng-Chun [1 ]
Yu, Roch-Chui [1 ]
机构：
[1] Natl Taiwan Univ, Grad Inst Food Sci & Technol, Taipei 10617, Taiwan
来源：
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2011年 / 721卷 / 02期
关键词：
Lactobacillus casei 01; Exopolysaccharides; 4-Nitroquinoline; 1-oxide; Anticytotoxicity; Comet assay; LACTIC-ACID BACTERIA; PROBIOTIC BACTERIA; COLON-CANCER; DNA-DAMAGE; IN-VITRO; PROLIFERATION; STRAINS; ANTIGENOTOXICITY; POLYSACCHARIDE; MECHANISMS;
D O I：
10.1016/j.mrgentox.2011.01.005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cell fractions including heat-treated cells, crude cell walls, intracellular extracts and exopolysaccharides (EPSs) obtained from Lactobacillus casei 01 were first studied for their effects on the proliferation of human intestinal epithelial cells, intestine 407 and the human colon cancer cell, HT-29. Their effects on the cytotoxicity of 4-nitroquinoline 1-oxide (4-NQO) against intestine 407 were further investigated. The results revealed that EPS exhibited the highest antiproliferation activity on HT-29 cells while the viability of intestine 407 cells was not affected by EPS at a concentration of 5-50 mu g/mL. It was also noted that all the cell fractions and EPS from L casei 01 reduced the cytotoxicity of 4-NQO against intestine 407 with EPS showing the highest anticytotoxic activity. Additionally, it was found that EPS might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells, thus reducing cytotoxicity of 4-NQO. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 162
页数：6
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