Anticancer Potential of L-Histidine-Capped Silver Nanoparticles against Human Cervical Cancer Cells (SiHA)

被引：8

作者：

Asik, Rajmohamed Mohammed ^{[1
]}

Manikkaraja, Chidhambaram ^{[1
]}

Surya, Karuppusamy Tamil ^{[1
]}

Suganthy, Natarajan ^{[2
]}

Aarthy, Archunan Priya ^{[3
]}

Mathe, Domokos ^{[4
,5
,6
]}

Sivakumar, Muthusamy ^{[7
]}

Archunan, Govindaraju ^{[1
,8
]}

Padmanabhan, Parasuraman ^{[9
,10
]}

Gulyas, Balazs ^{[9
,10
,11
]}

机构：

[1] Bharathidasan Univ, Dept Anim Sci, Tiruchirappalli 620024, Tamil Nadu, India

[2] Alagappa Univ, Dept Nanosci & Technol, Karaikkudi 630003, Tamil Nadu, India

[3] Rabindra Nath Tagore Med Coll, Dept Obstet & Gynecol, Udaipur 313001, Rajasthan, India

[4] Semmelweis Univ, Dept Biophys & Radiat Biol, H-1094 Budapest, Hungary

[5] CROmed Translat Res Ctr Ltd, H-1094 Budapest, Hungary

[6] Hungarian Ctr Excellence Mol Med HCEMM, In Vivo Imaging Adv Core Facil, H-1094 Budapest, Hungary

[7] Ariviya Technol Pvt Ltd, Pattukkottai 614602, Thanjavur, India

[8] Marudupandiyar Coll, Res, Thanjavur 613403, India

[9] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore 636921, Singapore

[10] Nanyang Technol Univ, Cognit Neuroimaging Ctr, 59 Nanyang Dr, Singapore 636921, Singapore

[11] Karolinska Inst, Dept Clin Neurosci, S-17176 Stockholm, Sweden

来源：

NANOMATERIALS | 2021年 / 11卷 / 11期

基金：

欧盟地平线“2020”;

关键词：

anticancer activity; L-histidine; silver nanoparticles; cervical cancer cells; apoptosis; IN-VITRO; ASSAY; AGGREGATION;

D O I：

10.3390/nano11113154

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This study reports the synthesis of silver nanoparticles using amino acid L-histidine as a reducing and capping agent as an eco-friendly approach. Fabricated L-histidine-capped silver nanoparticles (L-HAgNPs) were characterized by spectroscopic and microscopic studies. Spherical shaped L-HAgNPs were synthesized with a particle size of 47.43 & PLUSMN; 19.83 nm and zeta potential of -20.5 & PLUSMN; 0.95 mV. Results of the anticancer potential of L-HAgNPs showed antiproliferative effect against SiHa cells in a dose-dependent manner with an IC50 value of 18.25 & PLUSMN; 0.36 mu g/mL. Fluorescent microscopic analysis revealed L-HAgNPs induced reactive oxygen species (ROS) mediated mitochondrial dysfunction, leading to activation of apoptotic pathway and DNA damage eventually causing cell death. To conclude, L-HAgNPs can act as promising candidates for cervical cancer therapy.

引用

页数：17

共 40 条

[1] Anticancer Potential of Green Synthesized Silver Nanoparticles Using Extract of Nepeta deflersiana against Human Cervical Cancer Cells (HeLA) [J].

Al-Sheddi, Ebtesam S. ;

Farshori, Nida N. ;

Al-Oqail, Mai M. ;

Al-Massarani, Shaza M. ;

Saquib, Quaiser ;

Wahab, Rizwan ;

Musarrat, Javed ;

Al-Khedhairy, Abdulaziz A. ;

Siddiqui, Maqsood A. .

BIOINORGANIC CHEMISTRY AND APPLICATIONS, 2018, 2018

[2] Recent Advances in Silver and Gold Nanoparticles-Based Colorimetric Sensors for Heavy Metal Ions Detection: A Review [J].

Ali, Shujat ;

Chen, Xi ;

Shi, Wen ;

Huang, Guangzao ;

Yuan, Lei-ming ;

Meng, Liuwei ;

Chen, Shiliang ;

Zhonghao, Xie ;

Chen, Xiaojing .

CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY, 2023, 53 (03) :718-750

[3] Biological synthesis of silver nanoparticles using β-1, 3 glucan binding protein and their antibacterial, antibiofilm and cytotoxic potential [J].

Anjugam, Mahalingam ;

Vaseehara, Baskaralingam ;

Iswarya, Arokiadhas ;

Divya, Mani ;

Prabhu, Narayanan Marimuthu ;

Sankaranarayanan, Krishnasamy .

MICROBIAL PATHOGENESIS, 2018, 115 :31-40

[4]

Catalan J., 2014, Genotoxicity and DNA Repair: A Practical Approach

[5]

Daduang Jureerut, 2015, Asian Pac J Cancer Prev, V16, P169

[6] Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems [J].

Danaei, M. ;

Dehghankhold, M. ;

Ataei, S. ;

Davarani, F. Hasanzadeh ;

Javanmard, R. ;

Dokhani, A. ;

Khorasani, S. ;

Mozafari, M. R. .

PHARMACEUTICS, 2018, 10 (02)

[7] Impact of Environmental Conditions (pH, Ionic Strength, and Electrolyte Type) on the Surface Charge and Aggregation of Silver Nanoparticles Suspensions [J].

El Badawy, Amro M. ;

Luxton, Todd P. ;

Silva, Rendahandi G. ;

Scheckel, Kirk G. ;

Suidan, Makram T. ;

Tolaymat, Thabet M. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2010, 44 (04) :1260-1266

[8] Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles [J].

Foroozandeh, Parisa ;

Aziz, Azlan Abdul .

NANOSCALE RESEARCH LETTERS, 2018, 13

[9] Apoptotic efficacy of multifaceted biosynthesized silver nanoparticles on human adenocarcinoma cells [J].

George, Blassan Plackal Adimuriyil ;

Kumar, Neeraj ;

Abrahamse, Heidi ;

Ray, Suprakas Sinha .

SCIENTIFIC REPORTS, 2018, 8

[10] Dual-mode glucose nanosensor as an activatable theranostic platform for cancer cell recognition and cascades-enhanced synergetic therapy [J].

Hai, Xin ;

Zhu, Xueying ;

Yu, Kaixin ;

Yue, Shuzhen ;

Song, Weiling ;

Bi, Sai .

BIOSENSORS & BIOELECTRONICS, 2021, 192

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