Isolation, structural elucidation and immunomodulatory activity of fructans from aged garlic extract

被引:94
作者
Chandrashekar, Puthanapura M. [1 ]
Prashanth, Keelara V. Harish [2 ]
Venkatesh, Yeldur P. [1 ]
机构
[1] Cent Food Technol Res Inst, Dept Biochem & Nutr, Council Sci & Ind Res Lab, Mysore 570020, Karnataka, India
[2] Cent Food Technol Res Inst, Dept Meat Fish & Poultry Technol, Council Sci & Ind Res Lab, Mysore 570020, Karnataka, India
关键词
Aged garlic extract; Bioactive polysaccharides; Garlic fructans; C-13; NMR; Immunomodulation; Mitogenic activity; Macrophage activation; Phagocytosis; ALLIUM-SATIVUM; FRUCTOOLIGOSACCHARIDES; POLYSACCHARIDES; PROLIFERATION; OLIGOFRUCTOSE; ANTITUMOR; IMMUNITY; GROWTH;
D O I
10.1016/j.phytochem.2010.11.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Traditionally, garlic (Allium sativum) is known to be a significant immune booster. Aged garlic extract (AGE) possesses superior immunomodulatory effects than raw garlic; these effects are attributed to the transformed organosulfur compounds. AGE is also known to contain fructans; the amount of fructans in AGE represents a small fraction (0.22%) of the total fructans in raw garlic. In order to evaluate the biological activity of fructans present in AGE, both high molecular weight (>3.5 kDa; HF) and low molecular weight (<3 kDa; LF) fructans were isolated. The structures of purified HF and LF from AGE determined by H-1 NMR and C-13 NMR spectroscopy revealed that both have (2 -> 1) beta-D-fructofuranosyl bonds linked to a terminal glucose at the non-reducing end and beta-D-fructofuranosyl branching on its backbone. Biological activity of fructans was assessed by immunostimulatory activity using murine lymphocytes and peritoneal exudate cells (source of macrophages). Both HF and LF displayed mitogenic activity and activation of macrophages including phagocytosis. These activities were comparable to that of known polysaccharide immunomodulators such as zymosan and mannan. This study clearly demonstrates that garlic fructans also contribute to the immunomodulatory properties of AGE, and is the first such study on the biological effects of garlic fructans. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:255 / 264
页数:10
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