Genomic DNA amplification from a single bacterium

被引：223

作者：

Raghunathan, A ^{[1
]}

Ferguson, HR ^{[1
]}

Bornarth, CJ ^{[1
]}

Song, WM ^{[1
]}

Driscoll, M ^{[1
]}

Lasken, RS ^{[1
]}

机构：

[1] Mol Staging Inc, New Haven, CT USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 06期

关键词：

D O I：

10.1128/AEM.71.6.3342-3347.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.

引用

收藏

页码：3342 / 3347

页数：6

相关论文

共 7 条

[1] Comprehensive human genome amplification using multiple displacement amplification [J].

Dean, FB ;

Hosono, S ;

Fang, LH ;

Wu, XH ;

Faruqi, AF ;

Bray-Ward, P ;

Sun, ZY ;

Zong, QL ;

Du, YF ;

Du, J ;

Driscoll, M ;

Song, WM ;

Kingsmore, SF ;

Egholm, M ;

Lasken, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5261-5266

[2] Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification [J].

Dean, FB ;

Nelson, JR ;

Giesler, TL ;

Lasken, RS .

GENOME RESEARCH, 2001, 11 (06) :1095-1099

[3] Isothermal strand-displacement amplification applications for high-throughput genomics [J].

Detter, JC ;

Jett, JM ;

Lucas, SM ;

Dalin, E ;

Arellano, AR ;

Wang, M ;

Nelson, JR ;

Chapman, J ;

Lou, YI ;

Rokhsar, D ;

Hawkins, TL ;

Richardson, PM .

GENOMICS, 2002, 80 (06) :691-698

[4]

HANDYSIDE AH, 2004, MOL HUM REPROD AUG

[5] Unbiased whole-genome amplification directly from clinical samples [J].

Hosono, S ;

Faruqi, AF ;

Dean, FB ;

Du, YF ;

Sun, ZY ;

Wu, XH ;

Du, J ;

Kingsmore, SF ;

Egholm, M ;

Lasken, RS .

GENOME RESEARCH, 2003, 13 (05) :954-964

[6] Whole genome analysis of genetic alterations in small DNA samples using hyperbranched strand displacement amplification and array-CGH [J].

Lage, JM ;

Leamon, JH ;

Pejovic, T ;

Hamann, S ;

Lacey, M ;

Dillon, D ;

Segraves, R ;

Vossbrinck, B ;

González, A ;

Pinkel, D ;

Albertson, DG ;

Costa, J ;

Lizardi, PM .

GENOME RESEARCH, 2003, 13 (02) :294-307

[7] Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens [J].

Lasken, RS ;

Egholm, M .

TRENDS IN BIOTECHNOLOGY, 2003, 21 (12) :531-535