Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L.

被引：121
作者：
Gu, Jinyan [1 ]
Zhang, Haihui [1 ,2 ]
Yao, Hui [1 ]
Zhou, Jie [1 ]
Duan, Yuqing [1 ,2 ]
Ma, Haile [1 ,2 ]
机构：
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
[2] Jiangsu Univ, Inst Food Phys Proc, Zhenjiang 212013, Jiangsu, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2020年 / 235卷
关键词：
Sagittaria sagittifolia L. polysaccharides; Ultrasound-assisted extraction; Subcritical water extraction; Preliminary structure; Antioxidant and immunomodulatory activity; ULTRASOUND-ASSISTED EXTRACTION; PHYSICOCHEMICAL PROPERTIES; STRUCTURAL-CHARACTERIZATION; RHEOLOGICAL PROPERTIES; INHIBITORY-ACTIVITIES; FUNCTIONAL-PROPERTIES; BIOACTIVITY; PURIFICATION; DEGRADATION; FRACTIONS;
D O I：
10.1016/j.carbpol.2020.115939
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction. Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods. Furthermore, the antioxidant activity and immunostimulatory effects were investigated in vitro. Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios. In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU. SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.
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页数：11
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