CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons

被引：21

作者：

Cha, Caihui ^{[1
,2
,3
]}

Zhang, Jifeng ^{[3
]}

Ji, Zhisheng ^{[3
,4
]}

Tan, Minghui ^{[3
,4
]}

Li, Sumei ^{[3
]}

Wu, Fengming ^{[3
]}

Chen, Keen ^{[3
,5
]}

Gong, Sitang ^{[2
]}

Guo, Guoqing ^{[3
]}

Lin, Hongsheng ^{[4
]}

机构：

[1] Jinan Univ, Affiliated Hosp 1, Dept Pediat, Guangzhou 510630, Guangdong, Peoples R China

[2] Guangzhou Women & Childrens Med Ctr, Dept Pediat, Guangzhou 510120, Guangdong, Peoples R China

[3] Jinan Univ, Coll Med, Dept Anat, Guangzhou 510630, Guangdong, Peoples R China

[4] Jinan Univ, Affiliated Hosp 1, Dept Orthoped, Guangzhou 510630, Guangdong, Peoples R China

[5] Jinan Univ, Affiliated Hosp 1, Dept Neurosurg, Guangzhou 510630, Guangdong, Peoples R China

来源：

BRAIN RESEARCH BULLETIN | 2016年 / 124卷

基金：

中国国家自然科学基金;

关键词：

CRMP4; Actin; Dendritic development; Spine; mEPSC; Hippocampal neuron; AXON OUTGROWTH INHIBITION; MENTAL-RETARDATION; NEURITE OUTGROWTH; PHOSPHORYLATION; TUBULIN; CONE; MORPHOGENESIS; ARBORIZATION; ORGANIZATION; ENDOPHILIN;

D O I：

10.1016/j.brainresbull.2016.06.008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

CRMP family proteins (CRMPs) are critical for neurite outgrowth and maturation in the developing nervous system. However, the distinct roles of CRMP isoforms remain to be elucidated, especially in dendritic development. Here, we show that CRMP4 is sufficient and necessary for dendritic growth and maturation in cultured hippocampal neurons. Overexpression of CRMP4 promotes and genetic knockdown of CRMP4 inhibits the amount of dendritic tips, total dendritic length, spine density, and the frequency but not amplitude of miniature excitatory synaptic current. By GST-pulldown assay, we reveal that CRMP4 interacts with actin cytoskeleton by its C-terminal region, but not by N-terminal. Overexpression of actin-interacting region of CRMP4 promoted dendritic growth and maturation as CRMP4 wildtype. Taken together, these results suggest that CRMP4 is involved in dendritic development via the interaction with actin cytoskeleton in hippocampal neurons. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：286 / 294

页数：9

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