Hair-cycle-associated remodeling of the peptidergic innervation of murine skin, and hair growth modulation by neuropeptides

被引:83
作者
Peters, EMJ
Botchkarev, VA
Botchkareva, NV
Tobin, DJ
Paus, R
机构
[1] Univ Hamburg, Hosp Eppendorf, Dept Dermatol, D-20246 Hamburg, Germany
[2] Humboldt Univ, Charite, Dept Dermatol, Berlin, Germany
[3] Univ Bradford, Dept Biomed Sci, Bradford BD7 1DP, W Yorkshire, England
关键词
hair cycle; hair follicle; neuropeptide; calcitonin-gene-related peptide; peptide histidine methionine; substance P; vasoactive intestinal peptide;
D O I
10.1046/j.1523-1747.2001.01232.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
As the neuropeptide substance P can manipulate murine hair growth in vivo, we here further studied the role of sensory neuropeptides in hair follicle biology by determining the distribution and hair-cycle-dependent remodeling of the sensory innervation in C57BL/6 mouse back skin. Calcitonin-gene-related peptide, substance P, and peptide histidine methionine (employed as vasoactive intestinal peptide marker) were identified by immunohistochemistry, All of these markers immunolocalized to bundles of nerve fibers and to single nerve fibers, with distinct distribution patterns and major hair-cycle-associated changes. In the epidermis and around the distal hair follicle and the arrector pill muscle, only calcitonin-gene-related peptide immunoreactive nerve fibers were visualized, whereas substance P and peptide histidine methionine immunoreactive nerve fibers were largely restricted to the dermis and subcutis. Compared to telogen skin, the number of calcitonin-gene-related peptide, substance P, and peptide histidine methionine immunoreactive single nerve fibers increased significantly (p<0.01) during anagen, including around the bulge region (the seat of epithelial stem cells). Substance P significantly accelerated anagen progression in murine skin organ culture, whereas calcitonin-gene-related peptide and a substance-P-inhibitory peptide inhibited anagen (p<0.05). The inhibitory effect of calcitonin-gene-related peptide could be antagonized by coadministrating substance P. In contrast to substance P, calcitonin-gene-related peptide failed to induce anagen when released from subcutaneous implants. This might reflect a differential functional assignment of the neuropeptides calcitonin-gene-related peptide and substance P in hair growth control, and invites the use of neuropeptide receptor agonists and antagonists as novel pharmacologic tools for therapeutic hair growth manipulation.
引用
收藏
页码:236 / 245
页数:10
相关论文
共 54 条
  • [1] Interactions of the skin and nervous system
    Ansel, JC
    Armstrong, CA
    Song, I
    Quinlan, KL
    Olerud, JE
    Caughman, SW
    Bunnett, NW
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY SYMPOSIUM PROCEEDINGS, VOL 2, NO 1, AUGUST 1997, 1997, : 23 - 26
  • [2] SPECIFIC INDUCTION OF CAMP IN LANGERHANS CELLS BY CALCITONIN-GENE-RELATED PEPTIDE - RELEVANCE TO FUNCTIONAL-EFFECTS
    ASAHINA, A
    MORO, O
    HOSOI, J
    LERNER, EA
    XU, S
    TAKASHIMA, A
    GRANSTEIN, RD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) : 8323 - 8327
  • [3] A simple immunofluorescence technique for simultaneous visualization of mast cells and nerve fibers reveals selectivity and hair cycle - Dependent changes in mad cell - Nerve fiber contacts in murine skin
    Botchkarev, VA
    Eichmuller, S
    Peters, EMJ
    Pietsch, P
    Johansson, O
    Maurer, M
    Paus, R
    [J]. ARCHIVES OF DERMATOLOGICAL RESEARCH, 1997, 289 (05) : 292 - 302
  • [4] Botchkarev VA, 1997, J COMP NEUROL, V386, P379, DOI 10.1002/(SICI)1096-9861(19970929)386:3<379::AID-CNE4>3.0.CO
  • [5] 2-Z
  • [6] Hair cycle-dependent changes in adrenergic skin innervation, and hair growth modulation by adrenergic drugs
    Botchkarev, VA
    Peters, EMJ
    Botchkareva, NV
    Maurer, M
    Paus, R
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1999, 113 (06) : 878 - 887
  • [7] GROWTH OF THE HAIR
    CHASE, HB
    [J]. PHYSIOLOGICAL REVIEWS, 1954, 34 (01) : 113 - 126
  • [8] PEPTIDE IMMUNOCYTOCHEMISTRY IN AFFERENT NEURONS FROM LOWER GUT IN RATS
    CHERYCROZE, S
    BOSSHARD, A
    MARTIN, H
    CUBER, JC
    CHARNAY, Y
    CHAYVIALLE, JA
    [J]. PEPTIDES, 1988, 9 (04) : 873 - 881
  • [9] LABEL-RETAINING CELLS RESIDE IN THE BULGE AREA OF PILOSEBACEOUS UNIT - IMPLICATIONS FOR FOLLICULAR STEM-CELLS, HAIR CYCLE, AND SKIN CARCINOGENESIS
    COTSARELIS, G
    SUN, TT
    LAVKER, RM
    [J]. CELL, 1990, 61 (07) : 1329 - 1337
  • [10] CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVITY IN NERVE-FIBERS IN THE HUMAN-SKIN - RELATION TO FIBERS CONTAINING SUBSTANCE-P-LIKE, SOMATOSTATIN-LIKE AND VASOACTIVE-INTESTINAL-POLYPEPTIDE-LIKE IMMUNOREACTIVITY
    DALSGAARD, CJ
    JERNBECK, J
    STAINS, W
    KJARTANSSON, J
    HAEGERSTRAND, A
    HOKFELT, T
    BRODIN, E
    CUELLO, AC
    BROWN, JC
    [J]. HISTOCHEMISTRY, 1989, 91 (01) : 35 - 38