Hsp70 suppresses apoptosis in sympathetic neurones by preventing the activation of c-Jun

被引：26

作者：

Bienemann, A. S. ^{[1
]}

Lee, Y. B. ^{[1
]}

Howarth, J. ^{[1
]}

Uney, J. B. ^{[1
]}

机构：

[1] Univ Bristol, Henry Wellcome Labs Integrat Neurosci & Endocrino, Bristol BS1 3NY, Avon, England

来源：

JOURNAL OF NEUROCHEMISTRY | 2008年 / 104卷 / 01期

基金：

英国惠康基金;

关键词：

apoptosis; c-Jun; cytochrome c; Heat-shock protein 70; JNK; poly (ADP-ribose) polymerase-1;

D O I：

10.1111/j.1471-4159.2007.05006.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The anti-apoptotic effects of heat-shock protein (Hsp70) were assessed in SCG neurones following nerve growth factor (NGF) withdrawal. The results showed that the virally mediated expression of Hsp70 mirrored the effects of the c-Jun-N-terminal kinase (JNK) binding domain (JBD) of JNK interacting protein (an inhibitor of JNK and c-Jun activation) and suppressed the phosphorylation of c-Jun. Preventing c-Jun transcriptional activity subsequently led to reduced cytochrome c release and prevented caspase activation as indicated by a decrease in poly (ADP-ribose) polymerase-1 (PARP) cleavage. Together, these results show that Hsp70 is a highly effective inhibitor of apoptosis in sympathetic neurones and that it mediates this effect primarily by suppressing c-Jun transcriptional signalling.

引用

页码：271 / 278

页数：8

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