Development of methods for extraction and analytical characterization of carbon-based nanomaterials (nanoplastics and carbon nanotubes) in biological and environmental matrices by asymmetrical flow field-flow fractionation

被引:34
作者
Monikh, Fazel Abdolahpur [1 ]
Grundschober, Nadine [1 ]
Romeijn, Stefan [2 ]
Arenas-Lago, Daniel [3 ]
Vijver, Martina G. [1 ]
Jiskoot, Wim [2 ]
Peijnenburg, Willie J. G. M. [1 ,4 ]
机构
[1] Leiden Univ, Inst Environm Sci CML, POB 9518, NL-2300 RA Leiden, Netherlands
[2] Leiden Univ, Div BioTherapeut, Leiden, Netherlands
[3] Univ Vigo, Dept Plant Biol & Soil Sci, Vigo 36310, Spain
[4] Natl Inst Publ Hlth & Environm RIVM, Ctr Safety Subst & Prod, Bilthoven, Netherlands
基金
欧盟地平线“2020”;
关键词
Asymmetrical flow field-flow-fractionation; Multi-angle light scattering; Size distribution; Separation; Digestion; NATURAL ORGANIC-MATTER; VALENT COPPER NANOPARTICLES; TROPHIC TRANSFER; FOOD; MICROPLASTICS; SEPARATION; PRODUCTS; QUANTIFICATION; OPTIMIZATION; AGGREGATION;
D O I
10.1016/j.envpol.2019.113304
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Suitable methods and fit-for-purpose techniques are required to allow characterization of carbon-based nanomaterials (CB-NMs) in complex matrices. In this study, two methods were developed; a method for extraction and characterization of CB-NMs in biological media and a method for fractionation of natural organic matter (NOM) coated CB-NMs in environmental matrices. The former method was developed by extracting carbon nanotubes (CNTs: sized 0.75 x 3000 nm) and nanoplastics (sized 60, 200 and 600 nm) from eggshells and characterizing the extracted CB-NMs in terms of particle size distribution using asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle light scattering (MALS). The latter method was developed using AF4-MALS to fraction NOM-coated CNT (sized 0.75 x 3000 nm) and nanoplastics (sized 60, 200 and 300 nm) in a simulated natural surface water and provide information about the size distribution of the CB-NM-NOM complexes. The developed AF4-MALS method successfully fractioned the CB-NM-NOM complexes based on hydrodynamic size and provided the size distribution of the complexes. The NOM corona did not shift significantly the median size of the CB-NMs. It influenced however the size distribution of the nanoplastics and CNTs. The sample preparation method failed to extract the CNTs (recovery < 20%) from the matrices of the eggshells while being successful for extracting the nanoplastics (recoveries > 60%). The AF4-MALS fractogram showed that the extraction method did not significantly influence the size distribution of the nanoplastics of 60 and 200 nm size, whereas the peak of 600 nm nanoplastics shifted towards a smaller hydrodynamic size. In conclusion, the developed sample preparation method followed by the developed AF4-MALS method can be applied for extraction, separation and characterization of CB-NMs in biological and environmental matrices. Thus, the methods have a high potential to be methods of choice to investigate CB-NMs in future studies. (C) 2019 Elsevier Ltd. All rights reserved.
引用
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页数:9
相关论文
共 61 条
  • [1] Abdolahpur Monikh F, 2018, ENVIRON SCI TECHNOL, DOI [10.1021/acs.est8b03715, DOI 10.1021/ACS.EST8B03715]
  • [2] Interaction of zero valent copper nanoparticles with algal cells under simulated natural conditions: Particle dissolution kinetics, uptake and heteroaggregation
    Arenas-Lago, Daniel
    Monikh, Fazel Abdolahpur
    Vijver, Martina G.
    Peijnenburg, Willie J. G.
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 689 : 133 - 140
  • [3] Dissolution and aggregation kinetics of zero valent copper nanoparticles in (simulated) natural surface waters: Simultaneous effects of pH, NOM and ionic strength
    Arenas-Lago, Daniel
    Monikh, Fazel Abdolahpur
    Vijver, Martina G.
    Peijnenburg, Willie J. G. M.
    [J]. CHEMOSPHERE, 2019, 226 : 841 - 850
  • [4] Characterization of natural organic matter fractions separated by ultrafiltration using flow field-flow fractionation
    Assemi, S
    Newcombe, G
    Hepplewhite, C
    Beckett, R
    [J]. WATER RESEARCH, 2004, 38 (06) : 1467 - 1476
  • [5] Trophic transfer of microplastics in aquatic ecosystems: Identifying critical research needs
    Au, Sarah Y.
    Lee, Cindy M.
    Weinstein, John E.
    van den Hurk, Peter
    Klaine, Stephen J.
    [J]. INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT, 2017, 13 (03) : 505 - 509
  • [6] Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
    Avio, Carlo Giacomo
    Gorbi, Stefania
    Regoli, Francesco
    [J]. MARINE ENVIRONMENTAL RESEARCH, 2015, 111 : 18 - 26
  • [7] Beckett R, 2005, FIELD FLOW FRACTIONA
  • [8] Carbon nanotubes:: from production to functional composites
    Benito, A. M.
    Maser, W. K.
    Martinez, M. T.
    [J]. INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, 2005, 2 (1-2) : 71 - 89
  • [9] Bogatu C, 2016, J NANOTOXICOL NANOME, V1, P45, DOI [10.4018/jnn.2016010104, DOI 10.4018/JNN.2016010104]
  • [10] Measuring nanoparticles size distribution in food and consumer products: a review
    Calzolai, L.
    Gilliland, D.
    Rossi, F.
    [J]. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2012, 29 (08): : 1183 - 1193