Concomitant expression and combined localization of Ets-1 and VEGF in experimental diabetic rat's retina

被引：0

作者：

Du, Zhao-Jiang ^{[1
]}

Li, Peng ^{[2
]}

Hui, Yan-Nian ^{[1
]}

Wang, Bai-Ren ^{[3
]}

Duan, Xiao-Li ^{[3
]}

Zhang, Rui ^{[4
]}

机构：

[1] Fourth Mil Med Univ, Dept Ophthalmol, Xijing Hosp, Xian 710032, Shaanxi Prov, Peoples R China

[2] 451 Hosp Chinese PLA, Dept Ophthalmol, Xian 710054, Shaanxi Prov, Peoples R China

[3] Fourth Mil Med Univ, Inst Neurosci Chinese PLA, Xian 710032, Shaanxi Prov, Peoples R China

[4] Fourth Mil Med Univ, Tangdu Hosp, Dept Pharm, Xian 710038, Shaanxi Prov, Peoples R China

来源：

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2009年 / 2卷 / 03期

关键词：

Ets-1; VEGF; diabetic retina; angiogenesis; ENDOTHELIAL GROWTH-FACTOR; TRANSCRIPTION FACTOR ETS-1; VASCULAR-PERMEABILITY; IN-VIVO; NEOVASCULARIZATION; ANGIOGENESIS; CELLS; INHIBITION; FLT-1; MODEL;

D O I：

暂无

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

AIM: To investigate the characteristics of Ets-1 and VEGF expression and distribution in the experimental diabetic rat retina. METHODS: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ). At 4 weeks after STZ-injection, animals were sacrificed. Total proteins were isolated from retinas of experimental eyes and control eyes and assessed by Western blot analysis. Frozen cross sections of eyeballs with 14 mu m thickness were used to perform double immunofluorescence staining with anti-Ets-1 and anti-VEGF antibodies. RESULTS: Both Ets-1 and VEGF expression were up-regulated in the diabetic retina, the distribution of Ets-1 and VEGF was identical to each other, and the two proteins were almost localized in all retinal layers. CONCLUSION: Ets-1 may contribute to the pathologic progress of the diabetic retina induced by VEGF.

引用

页码：223 / 226

页数：4

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