Concomitant expression and combined localization of Ets-1 and VEGF in experimental diabetic rat's retina

被引：0
作者：
Du, Zhao-Jiang [1 ]
Li, Peng [2 ]
Hui, Yan-Nian [1 ]
Wang, Bai-Ren [3 ]
Duan, Xiao-Li [3 ]
Zhang, Rui [4 ]
机构：
[1] Fourth Mil Med Univ, Dept Ophthalmol, Xijing Hosp, Xian 710032, Shaanxi Prov, Peoples R China
[2] 451 Hosp Chinese PLA, Dept Ophthalmol, Xian 710054, Shaanxi Prov, Peoples R China
[3] Fourth Mil Med Univ, Inst Neurosci Chinese PLA, Xian 710032, Shaanxi Prov, Peoples R China
[4] Fourth Mil Med Univ, Tangdu Hosp, Dept Pharm, Xian 710038, Shaanxi Prov, Peoples R China
来源：
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2009年 / 2卷 / 03期
关键词：
Ets-1; VEGF; diabetic retina; angiogenesis; ENDOTHELIAL GROWTH-FACTOR; TRANSCRIPTION FACTOR ETS-1; VASCULAR-PERMEABILITY; IN-VIVO; NEOVASCULARIZATION; ANGIOGENESIS; CELLS; INHIBITION; FLT-1; MODEL;
D O I：
暂无
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
AIM: To investigate the characteristics of Ets-1 and VEGF expression and distribution in the experimental diabetic rat retina. METHODS: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ). At 4 weeks after STZ-injection, animals were sacrificed. Total proteins were isolated from retinas of experimental eyes and control eyes and assessed by Western blot analysis. Frozen cross sections of eyeballs with 14 mu m thickness were used to perform double immunofluorescence staining with anti-Ets-1 and anti-VEGF antibodies. RESULTS: Both Ets-1 and VEGF expression were up-regulated in the diabetic retina, the distribution of Ets-1 and VEGF was identical to each other, and the two proteins were almost localized in all retinal layers. CONCLUSION: Ets-1 may contribute to the pathologic progress of the diabetic retina induced by VEGF.
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页码：223 / 226
页数：4
相关论文
共 17 条
[1]   HYPOXIC REGULATION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR IN RETINAL CELLS [J].
AIELLO, LP ;
NORTHRUP, JM ;
KEYT, BA ;
TAKAGI, H ;
IWAMOTO, MA .
ARCHIVES OF OPHTHALMOLOGY, 1995, 113 (12) :1538-1544
[2]   SUPPRESSION OF RETINAL NEOVASCULARIZATION IN-VIVO BY INHIBITION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR (VEGF) USING SOLUBLE VEGF-RECEPTOR CHIMERIC PROTEINS [J].
AIELLO, LP ;
PIERCE, EA ;
FOLEY, ED ;
TAKAGI, H ;
CHEN, H ;
RIDDLE, L ;
FERRARA, N ;
KING, GL ;
SMITH, LEH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (23) :10457-10461
[3]   Vascular permeability in experimental diabetes is associated with reduced endothelial occludin content - Vascular endothelial growth factor decreases occludin in retinal endothelial cells [J].
Antonetti, DA ;
Barber, AJ ;
Khin, S ;
Lieth, E ;
Tarbell, JM ;
Gardner, TW .
DIABETES, 1998, 47 (12) :1953-1959
[4]   Inhibition of retinal neovascularisation by gene transfer of soluble VEGF receptor sFlt-1 [J].
Bainbridge, JWB ;
Mistry, A ;
De Alwis, M ;
Paleolog, E ;
Baker, A ;
Thrasher, AJ ;
Ali, RR .
GENE THERAPY, 2002, 9 (05) :320-326
[5]   Neural apoptosis in the retina during experimental and human diabetes - Early onset and effect of insulin [J].
Barber, AJ ;
Lieth, E ;
Khin, SA ;
Antonetti, DA ;
Buchanan, AG ;
Gardner, TW .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (04) :783-791
[6]   Transcriptomic analysis of the cardiac left ventricle in a rodent model of diabetic cardiomyopathy: molecular snapshot of a severe myocardial disease [J].
Glyn-Jones, Sarah ;
Song, Sarah ;
Black, Michael A. ;
Phillips, Anthony R. J. ;
Choong, Soon Y. ;
Cooper, Garth J. S. .
PHYSIOLOGICAL GENOMICS, 2007, 28 (03) :284-293
[7]   VASCULOTROPIN VASCULAR ENDOTHELIAL GROWTH-FACTOR IS AN AUTOCRINE GROWTH-FACTOR FOR HUMAN RETINAL-PIGMENT EPITHELIAL-CELLS CULTURED IN-VITRO [J].
GUERRIN, M ;
MOUKADIRI, H ;
CHOLLET, P ;
MORO, F ;
DUTT, K ;
MALECAZE, F ;
PLOUET, J .
JOURNAL OF CELLULAR PHYSIOLOGY, 1995, 164 (02) :385-394
[8]   In vivo evidence of angiogenesis induced by transcription factor ets-1 - Ets-1 is located upstream of angiogenesis cascade [J].
Hashiya, N ;
Jo, N ;
Aoki, M ;
Matsumoto, K ;
Nakamura, T ;
Sato, Y ;
Ogata, N ;
Ogihara, T ;
Kaneda, Y ;
Morishita, R .
CIRCULATION, 2004, 109 (24) :3035-3041
[9]  
Kappel A, 2000, BLOOD, V96, P3078
[10]   VASCULAR ENDOTHELIAL GROWTH-FACTOR VASCULAR-PERMEABILITY FACTOR EXPRESSION IN A MOUSE MODEL OF RETINAL NEOVASCULARIZATION [J].
PIERCE, EA ;
AVERY, RL ;
FOLEY, ED ;
AIELLO, LP ;
SMITH, LEH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (03) :905-909
12