Purification of phycocyanin from Arthrospira platensis by hydrophobic interaction membrane chromatography

被引：36

作者：

Lauceri, Rosaria ^{[1
]}

Zittelli, Graziella Chini ^{[2
]}

Maserti, Biancaelena ^{[3
]}

Torzillo, Giuseppe ^{[2
]}

机构：

[1] CNR, Inst Ecosyst Study, Largo Tonolli 50, I-28922 Verbania, VB, Italy

[2] CNR, Inst Ecosyst Study, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, FI, Italy

[3] CNR, Inst Sustainable Plant Protect, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, FI, Italy

来源：

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2018年 / 35卷

基金：

欧盟地平线“2020”;

关键词：

Spirulina; Phycocyanin; Allophycocyanin; Purification; Membrane chromatography; C-PHYCOCYANIN; SINGLE-STEP; ANTIOXIDANT ACTIVITY; SPIRULINA-PLATENSIS; SEPARATION; RECOVERY; PHYCOBILIPROTEINS; PROTEINS; FRACTIONATION; EXTRACTION;

D O I：

10.1016/j.algal.2018.09.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cyanobacterium Arthrospira platensis is an important source of phycocyanin, which has many commercial applications in foods, cosmetics and pharmaceutics. In this study, hydrophobic interaction membrane chromatography (HIMC) was used for the first time to separate phycocyanin from allophycocyanin and obtain analytical grade phycocyanin, avoiding packed bed (i.e., packed column) chromatography steps. The extraction of phycobiliproteins was carried out on freeze-dried biomass samples suspended in NaCl 0.1 M, and the phycobiliprotein crude extract was obtained by centrifuging the suspension to eliminate cell debris. The crude extract, after the addition of an appropriate amount of ammonium sulphate, was loaded on an ammonium sulphate responsive commercial hydrophilic polyvinylidene fluoride (PVDF) membrane for the HIMC purification process. Applying a two-step HIMC purification procedure, a phycocyanin purity ratio of 4.20 and a yield of 67.0% was attained.

引用

页码：333 / 340

页数：8

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