Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon

被引：72

作者：

Hara, Yuichi ^{[1
]}

Fujii, Taiga ^{[1
]}

Kashida, Hiromu ^{[1
]}

Sekiguchi, Koji ^{[1
]}

Liang, Xingguo ^{[1
]}

Niwa, Kosuke ^{[3
]}

Takase, Tomokazu ^{[3
]}

Yoshida, Yasuko ^{[3
,4
]}

Asanuma, Hiroyuki ^{[1
,2
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[2] CREST Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

[3] NGK INSULATORS, Nagoya, Aichi, Japan

[4] MEXT Innovat Res Ctr PME, Nagoya, Aichi, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 32期

关键词：

Cy3; DNA; excitonic interactions; fluorescent probes; molecular beacons; PEPTIDE NUCLEIC-ACIDS; REAL-TIME DETECTION; THIAZOLE ORANGE; THREONINOL NUCLEOTIDES; DNA; PROBES; HYBRIDIZATION; SELECTIVITY; STRATEGY; DYES;

D O I：

10.1002/anie.201001459

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Excitonic interaction was utilized to design a highly sensitive in-stem molecular beacon (ISMB) in which both a fluorophore and a quencher on D-threoninols are incorporated as a pseudo base pair (see scheme; optimized combination with Cy3 and modified Methyl Red). Minimization of the difference between λmax of the fluorophore and quencher maximized quenching efficiency. © 2010 Wiley-VCH Verlag GmbH S. Co. KCaA Weinheim.

引用

页码：5502 / 5506

页数：5

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