Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon

被引:71
作者
Hara, Yuichi [1 ]
Fujii, Taiga [1 ]
Kashida, Hiromu [1 ]
Sekiguchi, Koji [1 ]
Liang, Xingguo [1 ]
Niwa, Kosuke [3 ]
Takase, Tomokazu [3 ]
Yoshida, Yasuko [3 ,4 ]
Asanuma, Hiroyuki [1 ,2 ]
机构
[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[2] CREST Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan
[3] NGK INSULATORS, Nagoya, Aichi, Japan
[4] MEXT Innovat Res Ctr PME, Nagoya, Aichi, Japan
关键词
Cy3; DNA; excitonic interactions; fluorescent probes; molecular beacons; PEPTIDE NUCLEIC-ACIDS; REAL-TIME DETECTION; THIAZOLE ORANGE; THREONINOL NUCLEOTIDES; DNA; PROBES; HYBRIDIZATION; SELECTIVITY; STRATEGY; DYES;
D O I
10.1002/anie.201001459
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(Figure Presented) Excitonic interaction was utilized to design a highly sensitive in-stem molecular beacon (ISMB) in which both a fluorophore and a quencher on D-threoninols are incorporated as a pseudo base pair (see scheme; optimized combination with Cy3 and modified Methyl Red). Minimization of the difference between λmax of the fluorophore and quencher maximized quenching efficiency. © 2010 Wiley-VCH Verlag GmbH S. Co. KCaA Weinheim.
引用
收藏
页码:5502 / 5506
页数:5
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